CHARACTERIZATION OF VIBRIO-CHOLERAE NON-O1 SEROGROUPS OBTAINED FROM AN OUTBREAK OF DIARRHEA IN LIMA, PERU
CHARACTERIZATION OF VIBRIO-CHOLERAE NON-O1 SEROGROUPS OBTAINED FROM AN OUTBREAK OF DIARRHEA IN LIMA, PERU
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DOI：
10.1128/jcm.33.10.2715-2722.1995
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发表时间：
1995-10-01
影响因子：
9.4
通讯作者：
ECHEVERRIA, P
中科院分区：
文献类型：
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作者：
DALSGAARD, A;ALBERT, MJ;ECHEVERRIA, P
1994年2月，在秘鲁利马的一个疫苗试验研究区，在志愿者中发生了由非O 1型霍乱弧菌引起的腹泻暴发。临床上，95%的患者出现液体腹泻，无脱水或轻度脱水。血清群以O 10（21%）和O 12（65%）为主，多数菌株对多种抗菌药物敏感。用GM、神经节苷脂酶联免疫吸附试验（ELISA）检测58株V：霍乱非O 1菌株，均未发现与NAG-ST基因探针杂交或产生霍乱毒素的菌株。在所研究的58株V：霍乱非O 1菌株中，核糖体分型显示出10种不同的Bg/I：核糖体型。然而，核糖体分型显示，所有属于血清群O 12的分离株均表现出相同的核糖体分型，83%的血清群O 10分离株属于另一种相同的核糖体分型，因此显示核糖体分型和血清群之间具有良好的相关性。在选择用于毒力研究的一组O 10和O 12分离株中，如在Y1和HeLa细胞中所评估的，没有一个产生肠毒素，而大多数产生细胞毒素。如通过PCR测定所评估的，这些分离株对于编码闭锁小带毒素（Zot）的基因也是阴性的。所测试的分离株在HEp-2细胞测定中显示出强粘附和一定程度的侵袭。而在编码毒素共调节菌毛亚单位A抗原（tcpA）的基因的PCR测定中，没有一个分离株是阳性的。在可移除肠结扎成年兔腹泻模型中，当用10（10）个细菌细胞攻击兔时，O 10和O 12血清群分离株产生严重腹泻和偶尔死亡，在家兔肠袢试验中，当注射全培养物时，显示液体蓄积。血清群O 10和O 12分离株之间的毒力没有显著差异。本研究提供了进一步的证据，证明霍乱弧菌非O 1非O 139株通过一种尚未确定的机制对人类具有致病潜力，并且此类菌株可引起暴发。
In February 1994, an outbreak of diarrhea caused by non-Ol Vibrio cholerae occurred among volunteers in a vaccine trial study area in Lima, Peru, Clinically, 95% of the patients presented,vith liquid diarrhea with either no or mild dehydration, Serogrouping of 58 isolates recovered from diarrheal patients affected in the outbreak revealed seven different serogroups, with serogroups O10 (21%) and O12 (65%) being predominant, Most of these isolates were susceptible to a variety of antimicrobial agents. None of the 58 isolates hybridized with a DNA probe previously used to detect the gene encoding the heat-stable enterotoxin NAG-ST or produced cholera toxin as assessed by GM, ganglioside enzyme-linked immunosorbent assay, Ribotyping exhibited 10 different Bg/I: ribotype patterns among the 58 V: cholerae non Ol strains studied. However, ribotyping showed that all isolates belonging to serogroup O12 exhibited identical ribotypes and that 83% of the serogroup O10 isolates belonged to another identical ribotype, thus showing excellent correlation between ribotypes and serogroups. Among a group of O10 and O12 isolates selected for virulence studies, none produced enterotoxin whereas the majority produced a cytotoxin, as assessed in Y1 and HeLa cells, These isolates were also negative for the gene encoding zonula occludens toxin (Zot) as assessed by a PCR assay, The isolates tested showed strong adherence and some degree of invasion in the HEp-2 cell assay, whereas none of the isolates was positive in the PCR assay for the gene encoding the toxin coregulated pilus subunit A antigen (tcpA), In the removable intestinal tie adult rabbit diarrhea model, O10 and O12 serogroup isolates produced severe diarrhea and occasionally death when rabbits were challenged with 10(10) bacterial cells, Fluid accumulation was shown in the rabbit intestinal loop test when whole cultures were injected, No significant difference in virulence was shown between serogroup O10 and O12 isolates, This study provides further evidence that V cholerae non-Ol non-O139 strains have diarrhegenic potential for humans through a yet-undefined mechanism(s) and that such strains can cause outbreaks.