Adhesive resin induces apoptosis and cell-cycle arrest of pulp cells
Adhesive resin induces apoptosis and cell-cycle arrest of pulp cells
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DOI：
10.1177/154405910308200804
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发表时间：
2003-08-01
影响因子：
7.6
通讯作者：
Nör, JE
中科院分区：
文献类型：
--
作者：
Mantellini, MG;Botero, TM;Nör, JE
在牙髓附近或直接在牙髓上应用粘合剂树脂可诱导牙髓炎症和缺乏牙本质再生。我们推测，牙本质桥接的缺乏是由于粘性树脂诱导的细胞凋亡负责牙髓愈合和牙本质再生。将小鼠成牙本质细胞样细胞（MDPC-23）、未分化牙髓细胞（OD-21）或巨噬细胞（RAW 264.7）暴露于聚合的SingleBond 0-40秒。Annexin V和碘化丙啶试验表明，SingleBond诱导MDPC-23、OD-21和巨噬细胞凋亡。凋亡细胞的比例依赖于粘附树脂聚合的程度。粘附树脂诱导的牙髓细胞死亡与促凋亡半胱氨酸蛋白酶Caspase-3的激活有关。有趣的是，大多数暴露于粘附树脂但未发生细胞凋亡的细胞均表现出细胞周期停滞。我们的结论是，在体外的牙本质牙髓复合体的再生参与细胞的粘附树脂诱导细胞凋亡和细胞周期阻滞。
The application of an adhesive resin near or directly over the pulp was shown to induce pulp inflammation and lack of dentin regeneration. We hypothesize that the absence of dentin bridging is due to adhesive-resin-induced apoptosis of cells responsible for pulp healing and dentin regeneration. Mouse odontoblast-like cells (MDPC-23), undifferentiated pulp cells (OD-21), or macrophages (RAW 264.7) were exposed to SingleBond polymerized for 0-40 seconds. Annexin V and propidium iodide assays demonstrated that SingleBond induced apoptosis of MDPC-23, OD-21, and macrophages. The proportion of apoptotic cells was dependent on the degree of adhesive resin polymerization. Adhesive-resin-induced death of pulp cells was associated with activation of the pro-apoptotic cysteine protease Caspase-3. Interestingly, most cells exposed to adhesive resin that did not undergo apoptosis showed cell-cycle arrest. We conclude that an adhesive resin induces apoptosis and cell-cycle arrest of cells involved in the regeneration of the dentin-pulp complex in vitro.