Comparative transcriptome analysis of grapevine in response to copper stress.
Comparative transcriptome analysis of grapevine in response to copper stress.
复制标题
葡萄树响应铜胁迫的比较转录组分析
DOI：
10.1038/srep17749
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发表时间：
2015-12-17
影响因子：
4.6
通讯作者：
Fang J
中科院分区：
文献类型：
--
作者：
Leng X;Jia H;Sun X;Shangguan L;Mu Q;Wang B;Fang J
葡萄藤是世界上最重要的经济和广泛种植的水果作物之一。随着工业化和铜杀菌剂在葡萄工业中的广泛应用，铜胁迫和铜污染也是影响葡萄生产和果酒品质的因素。通过RNA-seq分析，3843个转录本在Cu胁迫下表达显著差异，其中上调1892个，下调1951个。在这项研究中，我们发现了许多已知的和新的cu诱导和抑制基因。对葡萄样品的生物学分析表明，外源Cu对葡萄叶绿素代谢和光合活性有影响。大多数ROS解毒系统，包括抗氧化酶、应激相关蛋白和次生代谢物被强烈诱导。同时，脱落酸在铜胁迫中起负调控作用，与乙烯、生长素、茉莉酸和油菜素内酯作用相反。本研究还发现了一组铜胁迫特异性激活基因，编码铜转运蛋白、p1b型atp酶和多药转运蛋白。总的来说，这项工作是为了在转录组水平上深入了解葡萄的铜调控和应激反应机制。本研究还可以提供一些遗传信息，帮助我们更好地管理葡萄园和选育抗铜葡萄品种。
Grapevine is one of the most economically important and widely cultivated fruit crop worldwide. With the industrialization and the popular application of cupric fungicides in grape industry, copper stress and copper pollution are also the factors affecting grape production and berry and wine quality. Here, 3,843 transcripts were significantly differently expressed genes in response to Cu stress by RNA-seq, which included 1,892 up-regulated and 1,951 down-regulated transcripts. During this study we found many known and novel Cu-induced and -repressed genes. Biological analysis of grape samples were indicated that exogenous Cu can influence chlorophylls metabolism and photosynthetic activities of grapevine. Most ROS detoxification systems, including antioxidant enzyme, stress-related proteins and secondary metabolites were strongly induced. Concomitantly, abscisic acid functioned as a negative regulator in Cu stress, in opposite action to ethylene, auxin, jasmonic acid and brassinolide. This study also identified a set of Cu stress specifically activated genes coding copper transporter, P1B-type ATPase, multidrug transporters. Overall, this work was carried out to gain insights into the copper-regulated and stress-responsive mechanisms in grapevine at transcriptome level. This research can also provide some genetic information that can help us in better vinery management and breeding Cu-resistant grape cultivars.