Lipids and ultrastructure of Thraustochytrium sp. ATCC 26185
Lipids and ultrastructure of Thraustochytrium sp. ATCC 26185
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DOI：
10.1007/s11745-997-0107-z
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发表时间：
1997-01-01
期刊：
影响因子：
1.9
通讯作者：
Dute, R
中科院分区：
文献类型：
--
作者：
Weete, JD;Kim, H;Dute, R
作为营养上重要的多不饱和脂肪酸二十二碳六烯酸 (DHA) 的潜在商业生产者的属的代表，对破囊壶菌 ATCC 26185 进行了研究，以确定其 DHA 生产和脂质成分的潜力。来自液体摇瓶培养物的细胞含有 32% (w/w) 脂质，其中 18% 是不可皂化的脂质。主要饱和脂肪酸（14:0和16:0）占总脂肪酸的59%，DHA在培养6天后高达25%。角鲨烯占不可皂化脂质的63%，胆固醇占总甾醇的41%。检测到真核微生物预期的磷脂，其中磷脂酰胆碱为主要磷脂，占总量的76%。该物种的超微结构与其他破囊壶菌属物种相似，只是细胞没有表面鳞片，并且含有不寻常的膜状结构，似乎与石油形成有关。
As a representative of a genus with species considered to be potential commercial producers of the nutritionally important polyunsaturated fatty acid docosahexaenoic acid (DHA), Thraustochytrium sp ATCC 26185 was investigated to determine its potential for DHA production and lipid composition. Cells from liquid shake cultures contained 32% (w/w) lipid, 18% of which was nonsaponifiable lipid. The major saturated fatty acids (14:0 and 16:0) comprised up to 59% of the total fatty acids, and DHA was up to 25% after 6 d incubation. Squalene represented 63% of the nonsaponifiable lipid, and cholesterol composed 41% of the total sterols. The phospholipids expected for eucaryotic microbes were detected with phosphatidylcholine as the major phospholipid at 76% of the total. The ultrastructure of this species was similar to other Thraustochytrium species except that the cells did not have surface scales and they contained unusual membrane-like structures that appeared to be associated with oil formation.