Characteristics of coliphage ECP4 and potential use as a sanitizing agent for biocontrol of Escherichia coli O157:H7
Characteristics of coliphage ECP4 and potential use as a sanitizing agent for biocontrol of Escherichia coli O157:H7
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DOI：
10.1016/j.foodcont.2013.04.043
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发表时间：
2013-12-01
期刊：
影响因子：
6
通讯作者：
Park, Jong-Hyun
中科院分区：
文献类型：
--
作者：
Lee, Young-Duck;Kim, Jin-Young;Park, Jong-Hyun
大肠杆菌O 157：H7是一种重要的人类致病菌，因为它产生多种毒素，如志贺毒素。从牛粪便中分离到的噬菌体ECP-4属于Siphoviridae科，以测试其作为E.大肠杆菌O 157：H7。大肠杆菌噬菌体ECP 4的爆发大小约为80 PFU/细胞，经过30-35分钟的潜伏期。大肠杆菌噬菌体ECP 4对70 ℃以上的温度敏感;然而，在70%乙醇中30分钟后，其稳定性略微降低至1-2 log PFU/ml。此外，在大肠杆菌噬菌体ECP 4上未检测到滋贺毒素基因。大肠杆菌噬菌体ECP 4对E.大肠杆菌O 157：H7在蔬菜汁中培养5 h后未检出。当大肠杆菌噬菌体ECP 4作用于形成生物膜的E. coliO 157：H7，大肠杆菌E. coli O 157：H7的酶解产物。新鉴定的大肠杆菌噬菌体ECP 4能有效地降低E. coli O 157：H7或其生物膜型。因此，大肠杆菌噬菌体ECP-4可能是一种有效的生鲜食品杀菌剂。大肠杆菌O 157：H7在生物膜环境中的表达。(C)2013爱思唯尔有限公司保留所有权利。
Escherichia coli O157:H7 is an important pathogenic bacterium to humans because it produces various toxins, such as shiga-toxin. Coliphage ECP4, which belongs to the Siphoviridae family, was isolated from bovine feces to test its utility as a potential agent for the biocontrol of E. coli O157:H7. The burst size of coliphage ECP4 was about 80 PFU/cell, after a latent period of 30-35 min. Coliphage ECP4 was susceptible to temperatures above 70 degrees C; however, its stability was slightly reduced to 1-2 log PFU/ml after 30 min in 70% ethanol. In addition, the shiga toxin gene was not detected on coliphage ECP4. Coliphage ECP4 inhibited the growth of E. coli O157:H7 in vegetable juice, and was not detected in cabbage after 5 h. When coliphage ECP4 was applied to biofilm-formed E. coli O157:H7, E. coli O157:H7 was efficiently reduced. The newly identified coliphage ECP4 might effectively reduce E. coli O157:H7 or its biofilmed-form. Therefore, the coliphage ECP4 might be an efficient sanitizer for fresh produce contaminated with E. coli O157:H7 in the biofilm environment. (C) 2013 Elsevier Ltd. All rights reserved.