Passive osmotic properties of in situ human articular chondrocytes within non-degenerate and degenerate cartilage
Passive osmotic properties of in situ human articular chondrocytes within non-degenerate and degenerate cartilage
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DOI：
10.1002/jcp.20294
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发表时间：
2005-07-01
影响因子：
5.6
通讯作者：
Hall, AC
中科院分区：
文献类型：
--
作者：
Bush, PG;Hall, AC
骨关节炎的特征在于许多因素，包括蛋白多糖损失、胶原硬度降低和软骨水合作用增加。软骨细胞肿胀也会发生，并与骨关节炎的程度相关，然而，原因尚不清楚，但可能与其被动渗透特性的改变有关。我们已经使用双光子共聚焦激光扫描显微镜来测量被动渗透特性的原位软骨细胞内相对非退化和退化的人胫骨平台软骨，并从相对非退化软骨分离的软骨细胞。从总共42名接受关节成形术的患者中取出附着骨的外植体，并通过肉眼和显微镜将其分为两组，0 + 1级和2 + 3级。两组间软骨水合作用显著增加（P< 0.05），然而，当介质渗透压在0-480 mOsm之间变化时，没有变化。原位软骨细胞的被动渗透行为（在4 ℃）在一定范围的培养基渗透压（类似于0-515 mOsm）内是相同的，然而，与非退化软骨中的那些相比，退化软骨和分离的软骨细胞中的细胞的最大溶胀更大。大多数原位软骨细胞的肿胀是由于软骨退变引起的间质渗透压降低所致。然而，有一小部分原位软骨细胞的体积超过了（≥ 2,500 μ m 3）根据间质渗透压降低预测的体积。这些结果表明，对于所研究的大多数细胞，相对非退化、退化和分离的细胞之间的被动软骨细胞体积的差异完全由细胞外渗透压摩尔浓度（180-515 mOsm）的变化来解释。
Osteoarthritis is characterized by many factors, including proteoglycan loss, decreased collagen stiffness, and increased cartilage hydration. Chondrocyte swelling also occurs, and correlates with the degree of osteoarthritis, however, the cause is unknown but might be related to alterations to their passive osmotic properties. We have used two-photon confocal laser scanning microscopy to measure the passive osmotic characteristics of in situ chondrocytes within relatively non-degenerate and degenerate human tibial plateau cartilage, and in chondrocytes isolated from relatively non-degenerate cartilage. Explants with bone attached were taken from a total of 42 patients undergoing arthroplasty and graded macroscopically and microscopically into two groups, grade 0 + 1 and grade 2 + 3. There was a significant increase in cartilage hydration between these two groups (P< 0.05), however, there was no change when medium osmolarity was varied over similar to 0-480 mOsm. The passive osmotic behavior of in situ chondrocytes (at 4 degrees C) was identical over a range of culture medium osmolarities (similar to 0-515 mOsm), however, the maximum swelling of cells within degenerate cartilage and isolated chondrocytes was greater compared to those in non-degenerate cartilage. The swelling in the majority of in situ chondrocytes was accounted for by the reduced interstitial osmolarity occurring with cartilage degeneration. There was, however, a small population of in situ chondrocytes whose volume was in excess (>= 2,500 mu m(3)) of that predicted from the decreased interstitial osmotic pressure. These results show that for the majority of cells studied, the differences in passive chondrocyte volume between relatively non-degenerate, degenerate, and isolated cells were entirely accounted for by changes to the extracellular osmolarity (180-515 mOsm).