SPR bio-sensor using white light source and OSA
SPR bio-sensor using white light source and OSA
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DOI：
10.1007/978-3-540-36841-0_209
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发表时间：
2007
期刊：
影响因子：
1.9
通讯作者：
Nyeon-Sik Eum;Ho-Jin Do;Do-Eok Kim;Shin-Won Kang
中科院分区：
文献类型：
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作者：
Nyeon-Sik Eum;Ho-Jin Do;Do-Eok Kim;Shin-Won Kang
在本研究中，我们制作了波长型表面电浆子共振感测器，以真实的实时量测宽频带内的微观折射率变化。我们根据沙门氏菌抗原-抗体反应和青霉素酶-青霉素反应来测量SPR现象，以应用于SPR免疫传感器。我们的实验使用了SAM（自组装单分子层）方法将沙门氏菌抗体和青霉素酶固定在每个传感器芯片上。此外，沙门氏菌抗原和青霉素倒入每个抗体固定的传感器室中。沙门氏菌抗体和抗原的浓度分别为50 µm/ml和5 × 107 cfu/ml。青霉素酶和青霉素的浓度为0.5mg/ml和10 mM。使用OSA测量每个实验的共振波长的偏移30分钟。我们可以知道沙门氏菌抗原-抗体反应在10分钟后饱和，青霉素酶-青霉素反应在15分钟后饱和。对沙门氏菌抗原抗体反应和青霉素酶-青霉素反应，共振波长的变化分别为0.26nm/min和0.8nm/min。
In this study, we fabricated wavelength type SPR (surface plasmon resonance) sensor to measure microscopic refractive index change in wide band by real time. We measured SPR phenomena according to salmonella antigen-antibody reaction and penicillinase - penicillin reaction to apply in SPR immunosensor. Our experiments used a SAM (self assembled monolayer) method to immobilize Salmonella antibody and penicillinase on each sensor chips. Also, Salmonella antigen and penicillin poured within each antibody immobilized sensor chambers. Concentrations of Salmonella antibody and antigen are 50 µm/ml and 5 × 107cfu/ml. Concentration of penicillinase and penicillin are 0.5 mg/ml and 10 mM. The shifts of resonance wavelength for each experiment were measured using OSA for 30 minutes. We could know that salmonella antigen-antibody reactions were saturated after 10 minutes and penicillinase - penicillin reactions were saturated after 15 minutes. The shift of resonance wavelength was varied about 0.26 nm/min for Salmonella antigen-antibody reaction and 0.8 nm/min for penicillinase - penicillin reaction.