5-Formyluracil targeted biochemical reactions with proteins inhibit DNA replication, induce mutations and interference gene expression in living cells
5-Formyluracil targeted biochemical reactions with proteins inhibit DNA replication, induce mutations and interference gene expression in living cells
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5-甲酰尿嘧啶与蛋白质发生靶向生化反应，抑制活细胞中的 DNA 复制、诱导突变并干扰基因表达
DOI：
10.1016/j.cclet.2021.02.036
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发表时间：
2021-02
影响因子：
9.1
通讯作者：
Zhou Xiang
中科院分区：
文献类型：
--
作者：
Zou Guangrong;Zhang Kaiyuan;Yang Wei;Liu Chaoxing;Fang Zhentian;Zhou Xiang
共价DNA -蛋白质交联是一种有毒的DNA损伤，它会干扰基本的生物学过程，从而导致严重的生物学后果，如基因组不稳定和蛋白质错误表达。5-甲酰基尿嘧啶（5-Formyluracil, 5fU）是DNA中的一种重要修饰，主要来源于氧化损伤，存在于多种细胞和组织中。我们报道了5fU介导的dna -蛋白偶联物可能存在于人类细胞中[Zhouet al. CCS Chem. 2(2020) 54-63]。我们现在的目标是探索其在体外和体内的潜在生物学效应。在本文中，我们首次报道了5fU介导的dna -肽或dna -蛋白偶联物（都被称为DPCs）可以抑制不同的聚合酶绕过或引起突变。然后，我们进一步研究了5fu介导的dpc对功能的影响，这些dpc出现在不同的基因表达组分中[在启动子序列或5 ' -非翻译区（UTR）]。这些结果可能有助于更广泛地了解dna -蛋白质相互作用以及与5fU相关的生物学功能。
Covalent DNA–protein cross-links are toxic DNA lesions that interfere with essential biological processes, which can cause serious biological consequences, such as genomic instability and protein misexpression. 5-Formyluracil (5fU) as an important modification in DNA, which is mainly from oxidative damage, exists in a variety of cells and tissues. We have reported that 5fU mediated DNA–protein conjugates could exist in human cells [Zhouet al. CCS Chem. 2 (2020) 54–63]. We now aimed to explore its potential biological effectsin vitroandin vivo. In this paper, we firstly reported that 5fU intermediated DNA–peptide or DNA–protein conjugates (both were called DPCs) could inhibit different polymerases bypass or cause mutations. Then we further investigated the functional impacts caused by 5fU-mediated DPCs, which appeared in different gene expression components [in the promoter sequence or 5′-untranslated regions (UTR)]. These results together may contribute to a broader understanding of DNA–protein interactions as well as the biological functions associated with 5fU.
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影响因子：
4.1
作者：
Pande P;Ji S;Mukherjee S;Schärer OD;Tretyakova NY;Basu AK
通讯作者：
Basu AK
影响因子：
15
作者：
Hardisty RE;Kawasaki F;Sahakyan AB;Balasubramanian S
通讯作者：
Balasubramanian S
影响因子：
4.1
作者：
Sanchez, AM;Minko, IG;Lloyd, RS
通讯作者：
Lloyd, RS
DOI：
10.1002/anie.201708286
发表时间：
2017-11-06
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Ji S;Shao H;Han Q;Seiler CL;Tretyakova NY
通讯作者：
Tretyakova NY
DNA蛋白交联：形成，结构身份和生物学结果。
影响因子：
18.3
作者：
Tretyakova NY;Groehler A 4th;Ji S
通讯作者：
Ji S