Rapid spectrophotometric determination of red and yellow isochromic carotenoid fractions in paprika and red pepper oleoresins
Rapid spectrophotometric determination of red and yellow isochromic carotenoid fractions in paprika and red pepper oleoresins
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DOI：
10.1021/jf010400l
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发表时间：
2001-08-01
影响因子：
6.1
通讯作者：
Mínguez-Mosquera, MI
中科院分区：
文献类型：
--
作者：
Hornero-Méndez, D;Mínguez-Mosquera, MI
基于两个特征波长的紫外-可见分光光度测定和朗伯-比尔定律在多组分混合物中的应用，建立了一种快速测定辣椒和油树脂中红(R)和黄(Y)等色类胡萝卜素的方法。波长472和508 nm被选为最适合同时定量色素丙酮提取物中的这些组分。在472 nm和508 nm处对两个色素组分(R，Y)的比吸收系数(E)进行了实验测定，得出了计算两个组分的浓度、总色素含量和两个组分之间的比例的公式。用该分光光度法测定等色组分的误差为
A rapid method has been developed for the determination of the red (R) and yellow (Y) isochromic carotenoid pigments fractions in paprika and oleoresins, based on UV-visible spectrophotometric measurement at two characteristic wavelengths and application of the Lambert-Beer law for multicomponent mixtures. The wavelengths 472 and 508 nm were selected as the most appropriate for simultaneous quantification of these fractions in the acetone extract of pigments. Experimental determination of the specific absorption coefficients (E) for the two pigment fractions (R, Y) at 472 and 508 nm yielded equations to calculate the concentration of the two fractions, the total pigment content, and the ratio between the two fractions. The error in the determination of the isochromic fractions by the proposed spectrophotometric method was