Recovery of photosynthesis and growth rate in green, blue–green, and diatom algae after exposure to atrazine
Recovery of photosynthesis and growth rate in green, blue–green, and diatom algae after exposure to atrazine
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DOI：
10.1002/etc.1988
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发表时间：
2012-11
影响因子：
4.1
通讯作者：
R. Brain;Joshua R. Arnie;J. Porch;Alan J. Hosmer
中科院分区：
文献类型：
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作者：
R. Brain;Joshua R. Arnie;J. Porch;Alan J. Hosmer
我们评估了脉冲暴露于阿特拉津后，绿色（Pseudokirchneriella subcapitata）、蓝绿色（Anabaena pelliculosa）和硅藻（舟形藻）的光合作用和生长速率的恢复。在24至72小时的生长期以建立用于测量光系统II（PSII）量子产率的足够信号强度所需的细胞密度之后，将藻类暴露于阿特拉津脉冲48小时，然后在对照培养基中进行48小时的恢复期。在0，3，6，12，24和48小时的曝光和恢复阶段，使用脉冲幅度调制荧光测定法测量光合作用，生长速率和细胞密度也同时测量在这些时间点。暴露于阿特拉津导致光合作用和生长的立即，但暂时的抑制，然而，这些影响是短暂的，在测试的藻类物种完全可逆。对于所有三种藻类，在阿特拉津从试验系统中去除后48小时，在任何处理浓度下均未检测到生长速率或PSII量子产率的统计学显著降低（p ≤ 0.05）。因此，在最高试验接触水平下的试验水平效应被确定为抑藻（可逆）。在生物化学和生理学方面，光合作用和生长速度立即恢复，在接触后数小时内达到对照水平。因此，鉴于阿特拉津的影响是暂时的，在代表本地种群的物种中是完全可逆的，因此，通常在美国中西部溪流中测量的阿特拉津脉冲暴露曲线不太可能导致初级生产发生具有生物学意义的变化。Environ.毒理学. 2012; 31：2572-2581。© 2012 SETAC
We evaluated the recovery of photosynthesis and growth rate in green (Pseudokirchneriella subcapitata), blue‐green (Anabaena flos‐aquae), and diatom (Navicula pelliculosa) algae after pulsed exposure to atrazine. Subsequent to a grow‐up period of 24 to 72 h to establish requisite cell density for adequate signal strength to measure photosystem II (PSII) quantum yield, algae were exposed to a pulse of atrazine for 48 h followed by a 48‐h recovery period in control media. Photosynthesis was measured at 0, 3, 6, 12, 24, and 48 h of the exposure and recovery phases using pulse amplitude modulation fluorometry; growth rate and cell density were also concomitantly measured at these time points. Exposure to atrazine resulted in immediate, but temporary, inhibition of photosynthesis and growth; however, these effects were transient and fully reversible in the tested species of algae. For all three algal species, no statistically significant reductions (p ≤ 0.05) in growth rate or PSII quantum yield were detected at any of the treatment concentrations 48 h after atrazine was removed from the test system. Effects at test levels up to the highest tested exposure levels were consequently determined to be algistatic (reversible). Both biochemically and physiologically, recovery of photosynthesis and growth rate occur immediately, reaching control levels within hours following exposure. Therefore, pulsed exposure profiles of atrazine typically measured in Midwestern U.S. streams are unlikely to result in biologically meaningful changes in primary production given that the effects of atrazine are temporary and fully reversible in species representative of native populations. Environ. Toxicol. Chem. 2012; 31: 2572–2581. © 2012 SETAC