The Fc epsilon RI beta subunit functions as an amplifier of Fc epsilon RI gamma-mediated cell activation signals
The Fc epsilon RI beta subunit functions as an amplifier of Fc epsilon RI gamma-mediated cell activation signals
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DOI：
10.1016/s0092-8674(00)81300-8
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发表时间：
1996-06-28
期刊：
影响因子：
64.5
通讯作者：
Kinet, JP
中科院分区：
文献类型：
--
作者：
Lin, SQ;Cicala, C;Kinet, JP
高亲和力IgE受体（Fc IgE RI）在过敏反应的起始中起着重要作用。Fc γ RI是多聚体，表达为α γ（2）三聚体或α β γ（2）四聚体。最近，β链基因的多态性与各种过敏表型的发展有关。到目前为止，β在Fc ε RI依赖性信号传导中的作用在很大程度上是未知的。出于这个原因，我们比较了各种野生型和突变型α-γ（2）和α-β-γ（2）Fc γ-RI复合物的信号特征。这些研究表明，γ二聚体作为一个自主激活模块，而β功能作为一个放大器，提供了5至7倍的增益，如测量Syk激活和钙动员。
The high affinity IgE receptor (Fc epsilon RI) plays a central role in the initiation of allergic responses. Fc epsilon RI is multimeric and is expressed as either alpha gamma(2) trimers or alpha beta gamma(2) tetramers. Recently, polymorphisms of the beta chain gene have been associated with the development of various allergic phenotypes. Until now, the role of beta in Fc epsilon RI-dependent signaling was largely unknown. For that reason, we compared the signaling characteristics of various wild-type and mutant alpha gamma(2) and alpha beta gamma(2) Fc epsilon RI complexes. These studies demonstrate that the gamma dimer functions as an autonomous activation module, while beta functions as an amplifier that provides a gain of 5- to 7-fold, as measured by Syk activation and calcium mobilization.