Interacting proteins Rtt109 and Vps75 affect the efficiency of non-homologous end-joining in Saccharomyces cerevisiae
Interacting proteins Rtt109 and Vps75 affect the efficiency of non-homologous end-joining in Saccharomyces cerevisiae
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DOI：
10.1016/j.abb.2007.11.001
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发表时间：
2008-01-15
影响因子：
3.9
通讯作者：
Golshani, Ashkan
中科院分区：
文献类型：
--
作者：
Jessulat, Matthew;Alamgir, Md;Golshani, Ashkan
DNA双链断裂(DSB)修复的关键途径之一是非同源末端连接(NHEJ)途径，它直接将DNA的两个断裂末端重新连接起来。通过质粒修复实验筛选，我们发现RTT109缺失菌株在酵母中对NHEJ的效率降低。这种缺失菌株在体内修复诱导的染色体DSB的效率也降低了。串联亲和纯化Rtt109回收的Vps75为物理相互作用蛋白。VPS75的缺失也被证明对NHEJ在质粒修复和染色体修复分析中的效率有影响。此外，RTT109和VPS75的缺失突变体对不同的DNA损伤剂都表现出超敏反应。我们的遗传相互作用分析支持RTT109在DNA损伤修复中的作用。我们认为Rtt109-Vps75相互作用蛋白对的一个功能是影响NHEJ在酵母中的效率。Vps75而不是Rtt109似乎也对使用同源重组的DSB修复效率有影响。(C)2007 Elsevier Inc.保留所有权利。
One of the key pathways for DNA double-stranded break (DSB) repair is the non-homologous end-joining (NHEJ) pathway, which directly re-ligates two broken ends of DNA. Using a plasmid repair assay screen, we identified that the deletion strain for RTT109 had a reduced efficiency for NHEJ in yeast. This deletion strain also had a reduced efficiency to repair induced chromosomal DSBs in vivo. Tandem-affinity purification of Rtt109 recovered Vps75 as a physical interacting protein. Deletion of VPS75 was also shown to have an effect on the efficiency of NHEJ in both the plasmid repair and the chromosomal repair assays. In addition, deletion mutants for both RTT109 and VPS75 showed hypersensitivity to different DNA damaging agents. Our genetic interaction analysis supports a role for RTT109 in DNA damage repair. We propose that one function of the Rtt109-Vps75 interacting protein pair is to affect the efficiency of NHEJ in yeast. Vps75 but not Rtt109 also seem to have an effect on the efficiency of DSB repair using homologous recombination. (C) 2007 Elsevier Inc. All rights reserved.