Limited access to ethanol increases the number of spontaneously active dopamine neurons in the posterior ventral tegmental area of nondependent P rats
Limited access to ethanol increases the number of spontaneously active dopamine neurons in the posterior ventral tegmental area of nondependent P rats
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DOI：
10.1016/j.alcohol.2010.02.009
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发表时间：
2010-05-01
期刊：
影响因子：
2.3
通讯作者：
Downey, David
中科院分区：
文献类型：
--
作者：
Morzorati, Sandra L.;Marunde, Rita L.;Downey, David
对嗜酒 (P) 大鼠的微透析实验表明，长期接触乙醇会增加伏隔核中多巴胺 (DA) 的细胞外水平。由于 DA 神经元活动有助于调节末端区域 DA 溢出，因此我们假设与仅饮用水的 P 大鼠相比，饮用乙醇的 P 大鼠后腹侧被盖区 (VTA) DA 神经元活动（放电频率、爆发活性和/或自发活跃 DA 神经元的数量）会增加。使用体内电生理技术来评估后 VTA 中单个 DA 神经元的活性。我们的研究结果表明，与仅消耗水的 P 大鼠相比，非依赖性 P 大鼠自愿摄入乙醇显着增加了后 VTA 中自发活跃的 DA 神经元的数量。两组之间的发射频率和爆发活动没有差异。这些结果表明，非依赖性 P 大鼠的中脑边缘 DA 系统发生了适应性变化，增加了 VTA 后部 DA 神经元的兴奋性，并增强了伏隔核神经末梢的 DA 释放。 (C) 2010 Elsevier Inc. 保留所有权利。
Microdialysis experiments in alcohol-preferring (P) rats have shown that chronic ethanol exposure increases extracellular levels of dopamine (DA) in the nucleus accumbens. Because DA neuronal activity contributes to the regulation of DA overflow in terminal regions, we hypothesized that posterior ventral tegmental area (VTA) DA neuronal activity (firing frequency, burst activity, and/or the number of spontaneously active DA neurons) would be increased in P rats consuming ethanol compared with P rats consuming only water. In vivo electrophysiological techniques were used to evaluate the activity of single DA neurons in the posterior VTA. Our findings show that voluntary ethanol intake by nondependent P rats significantly increased the number of spontaneously active DA neurons in the posterior VTA compared with P rats that consumed only water. Firing frequency and burst activity did not differ between the two groups. These results suggest that adaptive changes occur in the mesolimbic DA system of nondependent P rats to increase the excitability of posterior VTA DA neurons and enhance DA release from nerve terminals in the nucleus accumbens. (C) 2010 Elsevier Inc. All rights reserved.