Bacillus subtilis programs the differentiation of intestinal secretory lineages to inhibit Salmonella infection
Bacillus subtilis programs the differentiation of intestinal secretory lineages to inhibit Salmonella infection
复制标题
DOI：
10.1016/j.celrep.2022.111416
复制
发表时间：
2022-09-27
期刊：
影响因子：
8.8
通讯作者：
Li, Yuchen
中科院分区：
文献类型：
--
作者：
Hou, Qihang;Jia, Junpeng;Li, Yuchen
肠道微生物群对肠上皮细胞命运决定的作用尚未得到广泛研究。在这项研究中，我们探讨了枯草芽孢杆菌对程序性肠上皮分化的影响。我们发现枯草芽孢杆菌刺激肠道分泌细胞的分化。此外，枯草芽孢杆菌抑制Notch途径，减少hairy和split 1增强子的表达，从而将肠道干细胞分化向分泌细胞命运转变。此外，我们证明枯草芽孢杆菌对肠道分化的编程作用是 Toll 样受体 2 途径依赖性的。枯草芽孢杆菌与肠道内潘氏细胞和杯状细胞数量的增加有关。这导致抗菌肽的产生，以保护肠粘膜屏障免受鼠伤寒沙门氏菌的侵害。这项研究表明，枯草芽孢杆菌通过影响 Notch 通路信号传导来维持肠道屏障，从而有助于分泌细胞的分化。
The role of intestinal microbiota on fate determination of intestinal epithelial cells has not been extensively examined. In this study, we explore the effect of Bacillus subtilis on programmed intestinal epithelial differ-entiation. We find that B. subtilis stimulates the differentiation of intestinal secretory cells. Moreover, B. subtilis inhibits the Notch pathway to reduce the expression of hairy and enhancer of split 1, thereby shift-ing intestinal stem cell differentiation toward a secretory cell fate. Moreover, we demonstrate that the pro-gramming effect of B. subtilis on intestinal differentiation is Toll-like receptor 2 pathway dependent. B. subtilis is associated with increased numbers of Paneth and goblet cells in the intestine. This results in the production of antimicrobial peptides to protect the intestinal mucosal barrier against Salmonella typhimu-rium. This study demonstrates that B. subtilis contributes to the differentiation of secretory cells by affecting Notch pathway signaling to maintain the intestinal barrier.