SCANNING ELECTRON-MICROSCOPE STUDIES OF THE RAT MESENTERIC LYMPH-NODE WITH SPECIAL REFERENCE TO HIGH-ENDOTHELIAL VENULES AND HITHERTO UNKNOWN LYMPHATIC LABYRINTH
SCANNING ELECTRON-MICROSCOPE STUDIES OF THE RAT MESENTERIC LYMPH-NODE WITH SPECIAL REFERENCE TO HIGH-ENDOTHELIAL VENULES AND HITHERTO UNKNOWN LYMPHATIC LABYRINTH
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DOI：
10.1679/aohc.48.1
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发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
HE, Y
中科院分区：
文献类型：
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作者：
HE, Y
采用扫描电镜观察动脉灌注固定后大鼠肠系膜淋巴结的变化。主要观察高内皮小静脉（HEV）、内皮层和髓质。HEV分布于内皮层和外皮层滤泡间区，并延伸至边缘窦下方。记录了HEV内皮细胞的各种表面结构，从相当光滑到密集颗粒状。内皮细胞的细胞质脊集中在细胞顶端的中心。淋巴细胞大量附着在HEV的管腔表面。可以识别出许多淋巴细胞，可能是在HEV壁下或迁移后。在内皮层可见迄今未知的充满淋巴细胞的迷宫。这些由来自生发中心下方和HEV周围的衰减细胞排列的管状和囊状间隙流入髓窦。淋巴迷路与鼻窦的区别在于其缺乏网状细胞小梁和几乎完全含有小淋巴细胞。显然，HEV患者血液中的淋巴细胞大量迁移到迷路，并通过髓窦进入淋巴结的传出淋巴管。在髓窦中，发现一些与巨噬细胞相关的淋巴细胞延伸翼状突起，可能反映了它们的激活。偶见大的游离细胞呈层状突起，表面光滑。考虑到它们可能与被遮蔽的细胞相同（Kelly et al., 1978）。
Mesenteric lymph nodes of the rat were studied by scanning electron microscopy after arterial perfusion fixation. Observation was made mainly of the high endothelilal venules (HEV), inner cortex and medulla. The HEV were distribution in the inner cortex and in the interfollicular areas in the outer cortex, extending to closely beneath the marginal sinus. Various surface structures of the HEV endothelial cells were recorded, from quite smooth to densely granular. Special attention was paid to endothelial cells with cytoplasmic ridges concentrated at the center of the cell apex. Lymphocytes were numerously attached to the luminal surface of the HEV. Many lymphocytes presumably under and after emigration through the HEV wall could be recognized. Hitherto unknown labyrinths densely filled with lymphocytes were demonstrated in the inner cortex. These tubular and saccular spaces lined by attenuated cells originated from beneath the germinal centers and from around the HEV to pour into the medullary sinuses. The lymphatic labyrinths were differentiated from the sinuses by their lack of reticular cell trabeculae and by their containing almost exclusively small lymphcytes. Apparently, lymphocytes in the blood in HEV massively emigrate to the labyrinth, and via the medullary sinuses, enter the efferent lymphatics of the lymph node. In the medullary sinuses, some lymphocytes in association with a macrophages were found to extend wing-like processes, probably reflecting their activation. Large free cells extending laminar projections, but smooth in surface otherwise, were occasionally found. Their possible identity with the veiled cells (Kelly et al., 1978) was considered.