The effect of plant growth regulators and sucrose on the micropropagation and microtuberization of Dioscorea nipponica Makino
The effect of plant growth regulators and sucrose on the micropropagation and microtuberization of Dioscorea nipponica Makino
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DOI：
10.1007/s00344-005-0147-2
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发表时间：
2007-03-01
影响因子：
4.8
通讯作者：
Wang, Li
中科院分区：
文献类型：
--
作者：
Chen, Feng-Qing;Fu, Yang;Wang, Li
研究了蔗糖、植物生长调节剂、MS（Murashige 和 Skoog）和 ½MS 盐培养基配方对薯蓣芽培养、试管薯诱导和植株再生的影响。细胞分裂素N-苄基腺嘌呤(BA)在0.5-2.0 mg/l范围内仅在与生长素α-萘乙酸(NAA)联合使用时才显示出对试管薯诱导的强烈增强作用，其中NAA的效果从0.5 mg/l增加到2.0 mg/l。当 BA 浓度为 2.0 mg/l 且 NAA 浓度为 1.0 mg/l 时，补充有 3% (w/v) 蔗糖的 Murashige 和 Skoog 盐培养基产生最高的芽诱导频率 (86%)。 7% (w/v) 的蔗糖是对微薯生长最重要的单一培养基成分。最重的微块茎是在含有 1.0 mg/l BA 和 2.0 mg/l (0.073 g)，特别是含有 7% 蔗糖 (3.46 g) 的培养基上形成的。在含有 1/2MS、2% 蔗糖和 0.1% (w/v) 活性炭的培养基中，对于体外产生的芽，补充 1.0 mg/l BA 和 0.5 mg/l NAA 时，生根百分比最大（95%）；对于微块茎，补充 0.5 mg/l BA 和 NAA 时（100%），生根百分比最大。当从培养瓶中取出并转移到温室中的灭菌土壤中时，大多数硬化苗成活（1周后91％以上），1个月后适合大田种植。
The effects of sucrose, plant growth regulators, MS (Murashige and Skoog), and ½MS salt media formulations were investigated for the development of shoot cultures, microtuber induction, and plantlet regeneration inDioscorea nipponica. The cytokinin N-benzyladenine (BA) in the range of 0.5–2.0 mg/l showed strong enhancing effects on microtuber induction only when used in conjunction with the auxin alpha-naphthalene acetic acid (NAA), with the effect that NAA increased from 0.5 to 2.0 mg/l. Murashige and Skoog salt media supplemented with sucrose at 3% (w/v) gave the highest frequencies of shoot induction (86%) when BA was present at 2.0 mg/l and NAA at 1.0 mg/l. Sucrose at 7% (w/v) was the single most significant medium constituent for microtuber growth. The heaviest microtubers were formed on media containing 1.0 mg/l BA and 2.0 mg/l (0.073 g), especially with 7% sucrose (3.46 g). With media containing ½MS, 2% sucrose, and 0.1% (w/v) activated charcoal, the percentage of rooting was maximal when supplemented with 1.0 mg/l BA and 0.5 mg/l NAA for thein vitroproduced shoots (95%) and BA and NAA both at 0.5 mg/l for the microtubers (100%). When removed from culture flasks and transferred into sterilized soil in a greenhouse, most of the hardened plantlets survived (over 91% after 1 week), and they were suitable for field planting after 1 month.