Phylogenetic and evolutionary relationships among yellow fever virus isolated in Africa
Phylogenetic and evolutionary relationships among yellow fever virus isolated in Africa
复制标题
DOI：
10.1128/jvi.75.15.6999-7008.2001
复制
发表时间：
2001-08-01
影响因子：
5.4
通讯作者：
Barrett, ADT
中科院分区：
文献类型：
--
作者：
Mutebi, JP;Wang, HM;Barrett, ADT
先前对有限数量毒株的研究表明，非洲有两种黄热病（YF）病毒基因型，一种在西非，另一种在东非和中非。我们检查了来自非洲的代表不同国家和隔离时间的 38 种黄热病野生毒株的 prM/M 和部分 E 蛋白。对这些菌株的检查揭示了比之前报道的更复杂的遗传关系。总体而言，核苷酸替换范围为 0 至 25.8%，氨基酸替换范围为 0 至 9.18，使用简约和邻接算法的系统发育分析确定了五种不同的基因型：中/东非、东非、安哥拉、西非 I 和西非 II。观察到基因型内的广泛变异，西非基因型 II 和中/东非基因型的成员差异为 2.8% 或更少，而西非基因型 I 在核苷酸水平上的差异高达 6.8%。我们推测前两种基因型存在于地方性动物传播周期中，而后者由于定期人类流行病而在遗传上更加异质。安哥拉基因型的核苷酸序列与其他基因型的差异为 15.7% 至 23.0%，但在氨基酸水平上仅差异 0.4% 至 5.6%，这表明该基因型很可能在许多年前（在本研究中分析的其他东非/中非病毒株分离之前）与东非/中非的祖病毒 YF 病毒分化，并且已经独立进化。这些数据表明非洲存在多种 YF 病毒基因型，表明 YF 病毒在非洲不同地区独立进化。
Previous studies with a limited number of strains have indicated that there are two genotypes of yellow fever (YF) virus in Africa, one in west Africa and the other in east and central Africa, We have examined the prM/M and a portion of the E protein for a panel of 38 wild strains of YF virus from Africa representing different countries and times of isolation. Examination of the strains revealed a more complex genetic relationship than previously reported. Overall, nucleotide substitutions varied from 0 to 25.8% and amino acid substitutions varied from 0 to 9.18, Phylogenetic analysis using parsimony and neighbor-joining algorithms identified five distinct genotypes: central/east Africa, east Africa, Angola, west Africa I, and west Africa II. Extensive variation within genotypes was observed, Members of west African genotype II and central/east African genotype differed by 2.8% or less, while west Africa genotype I varied up to 6.8% at the nucleotide level, We speculate that the former two genotypes exist in enzootic transmission cycles, while the latter is genetically more heterogeneous due to regular human epidemics. The nucleotide sequence of the Angola genotype diverged from the others by 15.7 to 23.0% but only 0.4 to 5.6% at the amino acid level, suggesting that this genotype most likely diverged from a progenitor YF virus in east/central Africa many years ago, prior to the separation of the other east/central African strains analyzed in this study, and has evolved independently. These data demonstrate that there are multiple genotypes of YF virus in Africa and suggest independent evolution of YF virus in different areas of Africa.