Establishment of a dmp based maternal haploid induction system for polyploid Brassica napus and Nicotiana tabacum
Establishment of a dmp based maternal haploid induction system for polyploid Brassica napus and Nicotiana tabacum
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DOI：
10.1111/jipb.13244
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发表时间：
2022-04-11
影响因子：
11.4
通讯作者：
Chen, Shaojiang
中科院分区：
文献类型：
--
作者：
Zhong, Yu;Wang, Yuwen;Chen, Shaojiang
双单倍体（double haploid， DH）技术用于在一代内获得纯合子系，该技术显著加快了作物育种轨迹。传统上，体外培养是用来产生DHs的，但这种技术受到物种和基因型的限制。通过种子进行体内单倍体诱导（HI）在玉米中得到了广泛而有效的应用，最近已推广到其他几种作物中。本研究表明，在体内，异源多倍体（异源四倍体）甘蓝型油菜和烟草可通过DMP母源单倍体诱导基因突变引发HI。我们开发了一个DMP同源物的筛选管道，用于集群规则间隔的回文重复突变，结果表明，在甘蓝型油菜和烟草的多个基因型中，平均双单倍体诱导率分别为2.4%和1.2%。这些结果进一步证实了DMP基因在多倍体双子叶作物中的HI能力。DMP-HI系统为这些作物的育种提供了一种新的DH技术。该方法的成功和DMP基因在双果中的保存表明该技术在其他双果作物中的广泛适用性。
Doubled haploid (DH) technology is used to obtain homozygous lines in a single generation, a technique that significantly accelerates the crop breeding trajectory. Traditionally, in vitro culture is used to generate DHs, but this technique is limited by species and genotype recalcitrance. In vivo haploid induction (HI) through seed is widely and efficiently used in maize and was recently extended to several other crops. Here we show that in vivo HI can be triggered by mutation of DMP maternal haploid inducer genes in allopolyploid (allotetraploid) Brassica napus and Nicotiana tabacum. We developed a pipeline for selection of DMP orthologs for clustered regularly interspaced palindromic repeats mutagenesis and demonstrated average amphihaploid induction rates of 2.4% and 1.2% in multiple B. napus and N. tabacum genotypes, respectively. These results further confirmed the HI ability of DMP gene in polyploid dicot crops. The DMP-HI system offers a novel DH technology to facilitate breeding in these crops. The success of this approach and the conservation of DMP genes in dicots suggest the broad applicability of this technique in other dicot crops.