Membrane Insertion of New AMPA Receptors and LTP Induced by Glycine is Prevented by Blocking NR2A-Containing NMDA Receptors in the Rat Visual Cortex In Vitro
Membrane Insertion of New AMPA Receptors and LTP Induced by Glycine is Prevented by Blocking NR2A-Containing NMDA Receptors in the Rat Visual Cortex In Vitro
复制标题
体外阻断大鼠视觉皮层中含有 NR2A 的 NMDA 受体可防止甘氨酸诱导的新 AMPA 受体和 LTP 的膜插入
DOI：
10.2174/156720213804805927
复制
发表时间：
2013-02-01
影响因子：
2.1
通讯作者：
Wang, Jue
中科院分区：
文献类型：
--
作者：
Li, Yan-Hai;Wang, Jue
N-甲基-D-天冬氨酸受体（NMDA-R）的激活与各种形式的突触可塑性有关，这取决于所涉及的受体亚型。然而，NR 2A和NR 2B亚基的贡献甘氨酸诱导的长时程增强（LTP）的微型兴奋性突触后电流（mEPSC）在第II/III层锥体神经元的大鼠视皮层仍不清楚。本研究采用离体全细胞膜片钳技术研究了含NR 2A和NR 2B的NMDA受体在甘氨酸诱导的13-15日龄大鼠视皮层脑片LTP中的作用。我们发现，甘氨酸诱导的mEPSCs的LTP很容易诱导大鼠视皮层的第II/III层锥体神经元与甘氨酸。选择性NMDA-R拮抗剂D-APV可阻断甘氨酸诱导的LTP。此外，选择性含NR 2B的NMDA-R拮抗剂（Ro 25-6981）对甘氨酸诱导的LTP没有影响。然而，Zn ~（2+），一种电压非依赖性的含NR 2A的NMDA-R拮抗剂，阻止甘氨酸诱导的LTP。这些结果表明，甘氨酸诱导的大鼠视皮层第II/III层锥体神经元的LTP是NMDA-R依赖性的，需要含有NR 2A的NMDA-R，而不是含有NR 2B的NMDA-R。
N-methyl-D-aspartate receptors (NMDA-Rs) activation has been implicated in various forms of synaptic plasticity depending on the receptor subtypes involved. However, the contribution of NR2A and NR2B subunits in glycine-induced long-term potentiation (LTP) of miniature excitatory postsynaptic currents (mEPSCs) in layer II/III pyramidal neurons of the rat visual cortex remains unclear. The present study used whole-cell patch-clamp recordings in vitro to investigate the role of NR2A-containing and NR2B-containing NMDA-Rs in glycine-induced LTP in visual cortical slices from 13-15 day old rats. We found that glycine-induced LTP of mEPSCs was readily induced in layer II/III pyramidal neurons of the rat visual cortex with glycine. D-APV, a selective NMDA-R antagonist, blocked the glycine-induced LTP. Moreover, the selective NR2B-containing NMDA-R antagonists (Ro 25-6981) displayed no influence on the glycine-induced LTP. However, Zn2+, a voltage-independent NR2A-containing NMDA-R antagonist, prevented glycine-induced LTP. These results suggest that the glycine-induced LTP in layer II/III pyramidal neurons of the rat visual cortex is NMDA-R-dependent and requires NR2A-containing NMDA-Rs, not NR2B-containing NMDA-Rs.