A honeybee storage protein gene, hex 70a, expressed in developing gonads and nutritionally regulated in adult fat body
A honeybee storage protein gene, hex 70a, expressed in developing gonads and nutritionally regulated in adult fat body
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DOI：
10.1016/j.jinsphys.2008.03.009
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发表时间：
2008-05-01
影响因子：
2.2
通讯作者：
Gentile Bitondi, Marcia Maria
中科院分区：
文献类型：
--
作者：
Martins, Juliana Ramos;Franco Nunes, Francis Morais;Gentile Bitondi, Marcia Maria
在变态前的准备过程中，昆虫幼虫在血淋巴中储存了大量的蛋白质，主要是六聚蛋白。在蜜蜂幼虫中存在的四种六聚体中，HEX 70a表现出独特的发育模式，特别是因为它也存在于成虫中。在这里，我们报告的序列数据和实验证据表明，替代功能HEX 70a，除了其众所周知的作用，作为一种氨基酸资源在变态。hex 70 a基因由6个外显子组成，编码一条684个氨基酸的链，包含保守的血蓝蛋白N、M和C结构域。HEX 70a被归类为芳基蛋白，因为它含有超过15%的芳香族氨基酸。在成年工人的脂肪体中，hex 70a的表达被证明是一个营养有限的过程。然而，脂肪体不是hex 70a表达的唯一部位。在工蜂、蜂王和雄蜂的性腺发育过程中也检测到转录物和蛋白质亚基，表明它们在卵巢分化和睾丸成熟及功能中起作用。然而，在其假定的生殖作用中，HEX 70 a不同于卵黄蛋白，卵黄蛋白原，因为它在卵或胚胎中未被检测到。(C)2008爱思唯尔有限公司保留所有权利。
In preparing for metamorphosis, insect larvae store a huge amount of proteins in hemolymph, mainly hexamerins. Out of the four hexamerins present in the honeybee larvae, one, HEX 70a, exhibited a distinct developmental pattern, especially since it is also present in adults. Here, we report sequence data and experimental evidence suggesting alternative functions for HEX 70a, besides its well-known role as an amino acid resource during metamorphosis. The hex 70a gene consists of 6 exons and encodes a 684 amino acid chain containing the conserved hemocyanin N, M, and C domains. HEX 70a classifies as an arylphorin since it contains more than 15% of aromatic amino acids. In the fat body of adult workers, hex 70a expression turned out to be a nutrient-limited process. However, the fat body is not the only site for hex 70a expression. Both, transcript and protein subunits were also detected in developing gonads from workers, queens and drones, suggesting a role in ovary differentiation and testes maturation and functioning. In its putative reproductive role, HEX 70a however differs from the yolk protein, vitellogenin, since it was not detected in eggs or embryos. (C) 2008 Elsevier Ltd. All rights reserved.