Micronuclei Assay in Exfoliated Buccal Cells from Individuals Exposed to Arsenic in Argentina
Micronuclei Assay in Exfoliated Buccal Cells from Individuals Exposed to Arsenic in Argentina
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DOI：
10.1007/s00244-010-9607-1
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发表时间：
2011-08-01
影响因子：
4
通讯作者：
Ana Carballo, Marta
中科院分区：
文献类型：
--
作者：
Alicia Bartolotta, Susana;Gabriel Pacskowski, Matias;Ana Carballo, Marta
长期饮用含砷的水会影响人们的健康，并导致慢性氢砷中毒，这与不同类型癌症的发病率增加有关。为了确定与饮用水中不同程度的无机砷环境暴露相关的潜在遗传毒性风险，在阿根廷圣地亚哥德尔埃斯特罗的农村人群和布宜诺斯艾利斯的城市人群中，对脱落的口腔细胞中的微核（MN）频率进行了评估。圣地亚哥德尔埃斯特罗（La Firmeza和Santos Lugares地区）暴露组上皮细胞MN频率明显高于对照组（Monte Quemado和Urutau地区）（p = 0.0005）。就布宜诺斯艾利斯人群而言，纳瓦罗个体（暴露组）与对照组（布宜诺斯艾利斯自治市）相比表现出显著差异（p = 0.0002）。圣地亚哥德尔埃斯特罗和布宜诺斯艾利斯个体之间MN频率的比较表明，饮用水中砷的遗传毒性效应在农村和城市群体之间存在差异，可能是由于个体易感性是一个重要的发病因素。结果清楚地表明，口腔黏膜细胞锰含量测定是一种理想的方法，用于测量潜在的遗传风险与人类环境砷暴露。
Drinking arsenic (As)-laden water for a long time affects a population's health and leads to chronic hydroarsenicism, which is associated with an increased incidence of different types of cancer. To determine the potential genotoxic risk associated with different degrees of environmental exposure to inorganic As by way of drinking water, micronuclei (MN) frequency in exfoliated buccal cells was evaluated in Argentina among rural populations of Santiago del Estero and urban populations of Buenos Aires. The exposed group in Santiago del Estero (La Firmeza and Santos Lugares localities) showed a significant increase in MN frequency in epithelial cells compared with controls (Monte Quemado and Urutau localities) (p = 0.0005). With regard to the Buenos Aires groups, Navarro individuals (the exposed group) exhibited a significant difference compared with controls (Ciudad Autnoma de Buenos Aires) (p = 0.0002). Comparison of MN frequencies between Santiago del Estero and Buenos Aires individuals showed that genotoxic effects of As in drinking water exhibit variation between rural and urban groups, probably due to individual susceptibility being an important incidence factor. The results clearly show that MN assay in buccal mucosa cells is an ideal methodology with which to measure potential genetic risk related to environmental As exposure in humans.