A renewable electrochemical magnetic immunosensor based on gold nanoparticle labels.
A renewable electrochemical magnetic immunosensor based on gold nanoparticle labels.
复制标题
一种基于金纳米颗粒标记的可再生电化学磁性免疫传感器。
DOI：
10.1166/jnn.2005.178
复制
发表时间：
2005
影响因子：
--
通讯作者：
Yuehe Lin
中科院分区：
文献类型：
--
作者：
Guodong Liu;Yuehe Lin
利用磁珠和纳米金标记物，研制了一种基于颗粒的可再生电化学磁性免疫传感器。将抗体修饰的磁珠通过固定在传感器内部的磁铁附着在可再生的碳糊传感器表面。采用夹心免疫分析法将金纳米颗粒标记包覆在磁珠表面。高灵敏的电化学溶出分析提供了一种简单、快速的方法来定量捕获的纳米金颗粒示踪剂，并且避免了使用酶标记和底物。金纳米颗粒的溶出信号与样品溶液中目标免疫球蛋白的浓度有关。透射电子显微镜图像显示，金纳米粒子通过夹心免疫反应成功地被包裹在磁珠的表面。对磁性小球的负载量、金纳米粒子的用量、免疫反应时间等免疫分析参数进行了优化。在最佳实验条件下，该方法的检出限为0.02微克/毫升。这种基于粒子的电化学磁性免疫传感器可以通过使用多种无机金属纳米粒子示踪剂来方便地同时并行检测多种蛋白质，有望为疾病诊断和生物安全开辟新的机会。
A particle-based renewable electrochemical magnetic immunosensor was developed by using magnetic beads and gold nanoparticle labels. Anti-IgG antibody-modified magnetic beads were attached to a renewable carbon paste transducer surface by magnet that was fixed inside the sensor. Gold nanoparticle labels were capsulated to the surface of magnetic beads by sandwich immunoassay. Highly sensitive electrochemical stripping analysis offers a simple and fast method to quantify the capatured gold nanoparticle tracers and avoid the use of an enzyme label and substrate. The stripping signal of gold nanoparticles is related to the concentration of target IgG in the sample solution. A transmission electron microscopy image shows that the gold nanoparticles were successfully capsulated to the surface of magnetic beads through sandwich immunoreaction events. The parameters of immunoassay, including the loading of magnetic beads, the amount of gold nanoparticle conjugate, and the immunoreaction time, were optimized. The detection limit of 0.02 microg ml(-1) of IgG was obtained under optimum experimental conditions. Such particle-based electrochemical magnetic immunosensors could be readily used for simultaneous parallel detection of multiple proteins by using multiple inorganic metal nanoparticle tracers and are expected to open new opportunities for disease diagnostics and biosecurity.