Biochemical efficacy of vitamin D in ameliorating endocrine and metabolic disorders in diabetic rats
Biochemical efficacy of vitamin D in ameliorating endocrine and metabolic disorders in diabetic rats
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DOI：
10.3109/13880209.2013.854812
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发表时间：
2014-04
影响因子：
3.8
通讯作者：
K. Sadek;H. Shaheen
中科院分区：
文献类型：
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作者：
K. Sadek;H. Shaheen
摘要背景：由于维生素D(VitD)在内分泌系统中的生化作用，特别是其潜在的抗炎和免疫调节作用，其在糖尿病预防和控制中的潜在作用越来越受到人们的关注。目的：本研究评价维生素D在避免这两种类型糖尿病的不良反应方面的潜在疗效。材料和方法：雄性Wistar大鼠50只，随机分为5组：安慰剂组；非遗传性1型糖尿病大鼠模型(T1D)，单次注射链脲佐菌素(STZ；65 mg/kg，ip)；非遗传性2型糖尿病大鼠模型(T2D)，短期高脂饮食后单次小剂量STZ(35 mg/kg，ip)；第4、5组，分别于诱导后3d灌胃Vit D(10IU/kg)，连续3天。结果：维生素D(10IU/kg/60d)显著降低空腹血糖、酮症酸中毒(降低非酯化脂肪酸和β-羟基丁酸)、促炎性白介素6、糖化血红蛋白，降低胰岛素抵抗指数33%。有趣的是，维生素D显著提高了T1d的空腹血浆胰岛素、T1d和T2D的血钙水平、胰岛素敏感性指数和β细胞功能指数(P<0.05)。讨论与结论：维生素D可能通过增加胰岛素分泌和敏感性，改善β细胞功能，减少促炎细胞因子的数量和胰岛素抵抗，从而改善糖尿病的不良生化影响。
Abstract Context: Due to the biochemical role of vitamin D (Vit D) in the endocrine system, especially its potential anti-inflammatory and immune-modulating properties, there is an increased interest in its potential role in the prevention and control of diabetes mellitus. Objective: This study evaluated the potential therapeutic efficacy of Vit D in averting the detrimental effects of both types of diabetes mellitus. Materials and methods: A total of 50 male Wistar rats were allotted into five groups: a placebo group; a nongenetic model of type 1 diabetes in rats (T1D), injected with a single dose of streptozotocin (STZ; 65 mg/kg, ip); a nongenetic model of type 2 diabetes in rats (T2D), given a short-term high-fat diet followed by a single low dose of STZ (35 mg/kg, ip); fourth and fifth groups that were gastrogavaged with Vit D (10 IU/kg) three days after the induction of T1D and T2D, respectively, which was continued daily throughout the experiment. Results: Vit D (10 IU/kg/60 days) significantly (p < 0.05) decreased fasting plasma glucose, ketoacidosis (decreased non-esterified fatty acid and β-hydroxyl butyric acid), pro-inflammatory interleukin-6, HbA1c in T1D and T2D and insulin resistance index by 33% in T2D. Interestingly, Vit D significantly (p < 0.05) increased fasting plasma insulin by 144% in T1D, plasma Ca level, insulin sensitivity index, and β-cell function index in T1D and T2D. Discussion and conclusion: Vit D ameliorated the deleterious biochemical impact of diabetes mellitus, likely by increasing insulin secretion and sensitivity, ameliorating the β-cell function, and decreasing the number of pro-inflammatory cytokines and insulin resistance.