Effect of dietary graded resistant potato starch levels on growth performance, plasma cytokines concentration, and intestinal health in meat ducks
Effect of dietary graded resistant potato starch levels on growth performance, plasma cytokines concentration, and intestinal health in meat ducks
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日粮分级抗性马铃薯淀粉水平对肉鸭生长性能、血浆细胞因子浓度和肠道健康的影响
DOI：
10.3382/ps/pez186
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发表时间：
2019-09-01
期刊：
影响因子：
4.4
通讯作者：
Zeng, Q. F.
中科院分区：
文献类型：
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作者：
Qin, S. M.;Zhang, K. Y.;Zeng, Q. F.
本研究旨在探讨饲粮不同水平生马铃薯淀粉（RPS）对1 ~ 35日龄肉鸭生长性能、血浆细胞因子浓度、回肠屏障功能和盲肠短链脂肪酸（SCFA）浓度的影响。本研究包括两个实验。在实验中（Exp.）1、选用16只35日龄肉鸭，通过灌胃给药，研究RPS对肉鸭AME的影响。结果表明，RPS对肉鸭的AME值为2.76kcal/g。在Exp.选用600只1日龄雏鸭，随机分为5组，每组10只，分别饲喂0（对照）、6、12、18和24%RPS的等氮等能日粮。在14和35天采集样品。无论是生长性能，也不是回肠参数（长度，重量和pH值）在14和35 D的饮食RPS的影响。然而，粘膜厚度（14 D），绒毛高度（除了18% RPS在14 D），和绒毛高度：隐窝深度比（14和35 D）的回肠增加12和18% RPS饮食相比，0% RPS饮食。同时，12% RPS组血浆IL-1 β和IL-6（14 D）等促炎因子水平降低，12%（14 D除外）和18% RPS组肿瘤坏死因子α水平降低。与对照组相比，添加18%RPS的日粮显著提高了14 D时鸡回肠粘膜黏蛋白2基因的表达，12%RPS显著提高了鸡回肠粘膜紧密连接蛋白（包括闭锁小带1和Claudin 1）mRNA的表达（14 D除外）。此外，饲喂12%RPS日粮的鸭盲肠中乙酸、丙酸和丁酸的浓度高于其他组。这些结果表明，12和/或18%的RPS的饮食增加盲肠SCFA的浓度，随后增强屏障功能，改善肠道健康的回肠14日龄和35日龄肉鸭。
The objective of the present study was to investigate the effect of dietary graded raw potato starch (RPS) levels on growth performance, plasma cytokines concentration, ileal barrier function, and cecal short-chain fatty acids (SCFA) concentration in meat ducks from 1 to 35 D of age. This study included 2 experiments. In experiment (Exp.) 1, sixteen 35-day-old meat ducks were used to evaluate the AME of RPS by orogastric administration. Results showed the AME value of RPS on ducks is 2.76 kcal/g. In Exp. 2, a total of 600 one-day-old ducklings were randomly assigned to 5 isonitrogenous and isoenergetic dietary treatments that included 0 (control), 6, 12, 18, and 24% RPS, respectively. Samples were collected at both of 14 and 35 D. Neither growth performance nor ileal parameters (length, weight, and pH) at both of 14 and 35 D was affected by dietary RPS. However, the mucosal thickness (14 D), villus height (except for 18% RPS at 14 D), and the villus height: crypt depth ratio (14 and 35 D) of the ileum were increased in the 12 and 18% RPS diets when compared to 0% RPS diet. Meanwhile, proinflammatory factors such as plasma interleukin (IL)-1 beta and IL-6 (14 D) reduced in 12% RPS diet and tumor necrosis factor alpha decreased in 12% (except for 14 D) and 18% RPS groups. When compared with the control group, diets with 18% RPS significantly increased mucin 2 gene expression at 14 D, and 12% RPS elevated the mRNA expression of tight junction proteins including Zonula occludens-1 and Claudin 1 (except for 14 D) in the ileal mucosa of birds. Furthermore, ducks fed 12% RPS diet had higher concentrations of acetate, propionate, and butyrate in cecal digesta than other groups. These findings indicated that diets with 12 and/or 18% RPS increased the cecal SCFA concentration, which subsequently enhanced the barrier function and improved intestinal health in the ileum for 14 and 35-day-old meat ducks.