Bio-artificial pleura using an autologous dermal fibroblast sheet
Bio-artificial pleura using an autologous dermal fibroblast sheet
复制标题
DOI：
10.1038/s41536-017-0031-2
复制
发表时间：
2017-10-11
影响因子：
7.2
通讯作者：
Yamato, Masayuki
中科院分区：
文献类型：
--
作者：
Kanzaki, Masato;Takagi, Ryo;Yamato, Masayuki
肺切除术后观察到漏气（AL），如果没有适当的治疗，可能会产生严重的并发症。AL预防是肺切除术后管理患者的关键目标。本研究应用自体真皮成纤维细胞片（DFS）闭合AL。为了凝闭患有多发性大疱的44岁男性患者的AL，手术切除了5 x 15 mm的皮肤切片。从该皮肤样本中分离原代真皮成纤维细胞并培养4周以产生DFS，在培养10天后收获DFS。通过手术放置这些自体DFS完全封闭AL。DFS被认为是一种耐用的长期AL密封剂，表现出必要的柔韧性、弹性、耐久性、生物相容性和可用性，从而实现可靠的AL闭合。DFS应该被证明是一个非常有用的组织工程胸膜替代品。
Air leaks (ALs) are observed after pulmonary resections, and without proper treatment, can produce severe complications. AL prevention is a critical objective for managing patients after pulmonary resection. This study applied autologous dermal fibroblast sheets (DFS) to close ALs. For sealing ALs in a 44-year-old male human patient with multiple bullae, a 5 x 15-mm section of skin was surgically excised. From this skin specimen, primary dermal fibroblasts were isolated and cultured for 4 weeks to produce DFSs that were harvested after a 10-day culture. ALs were completely sealed using surgical placement of these autologous DFSs. DFS were found to be a durable long-term AL sealant, exhibiting requisite flexibility, elasticity, durability, biocompatibility, and usability, resulting reliable AL closure. DFS should prove to be an extremely useful tissue-engineered pleura substitute.