Prevalence of some herpesviruses in gingival crevicular fluid
Prevalence of some herpesviruses in gingival crevicular fluid
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DOI：
10.1016/j.jcv.2005.03.001
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发表时间：
2005-10-01
影响因子：
8.8
通讯作者：
Marin, J
中科院分区：
文献类型：
--
作者：
Klemenc, P;Skaleric, U;Marin, J
工作背景：疱疹病毒是一种与人类共同进化的古老病原体，它与许多疾病有着病原学上的联系，从无症状性疾病到致癌性疾病乃至致死性疾病。其中一些在人类牙周病的发病机制中也起着重要作用。目的：本研究旨在确定龈沟液（GCF）中EB病毒（EBV）、人类疱疹病毒6型（HHV-6）、人类疱疹病毒8型（HHV-8）和人类巨细胞病毒（HCMV）的患病率，最终，以发现特定病毒类型与牙周炎中重要的临床参数之间的相关性，如菌斑指数（PI）、牙龈指数（GI）和探诊深度（PD）。采用聚合酶链反应（PCR）和限制性内切酶酶切鉴定EBV、HHV-6、HHV-8和HCMV的存在。在66例牙周病患者龈沟液中，29例检出EB病毒牙周炎组和健康对照组均未检出HHV-8，HHV-6型16例（24.2%），HCMV型2例（3%）。PI和GI较高者和PD中等者（PD = 3-6 mm）阳性率较高。在HHV-6阳性标本中，仅发现A型变异株，而在EBV阳性标本中，发现A型和B型，并存在两型混合感染。结论：疱疹病毒感染与牙周炎有一定的相关性。(C)2005 Elsevier B. V.保留所有权利。
Background: The herpesviruses, ancient pathogens which have co-evoluted with human, are etiologically associated with a number of diseases, from asymptomatic to oncogenic and mortal diseases. It seems that some of them have also an important role in the pathogenesis of human periodontal disease.Objective: This study aimed to determine the prevalence of Epstein-Barr virus (EBV), human herpesvirus 6 (HHV-6), human herpesvirus 8 (HHV-8) and human cytomegalovirus (HCMV) in gingival crevicular fluid (GCF) and, eventually, to find the correlation between specific virus types and clinical parameters which are important in periodontitis, like plaque index (PI), gingival index (GI) and probing depth (PD).Study design: A polymerase chain reaction (PCR) and digestion of PCR products with restriction endonuclease were employed to identify the presence of EBV, HHV-6, HHV-8 and HCMV.Results: Out of 66 samples of GCF taken from the patients with periodontal disease, EBV was found in 29 (43.9%), HHV-6 in 16 (24.2%) and HCMV in 2 (3%) samples, while in the samples of healthy persons, these viruses were not found. HHV-8 was detected neither in the patients with periodontitis nor in healthy control group. More positive results were found in clinical samples taken from people with higher PI and GI and in the samples taken from the patients with medium PD (PD = 3-6 mm). In all HHV-6 positive samples, we found only variant A; as for EBV positive samples, type A and type B were identified and also co-infection with the two types. It seems that there is a correlation between PI, PD and EBV types, but no correlation was found between EBV types and GI or HHV-6 types and PI, PD, GI.Conclusions: The present findings confirm some association between herpesviruses and human periodontitis. (C) 2005 Elsevier B.V. All rights reserved.