Synthetic Blood Substitute Utilizing Hemoglobin/Oil/Water Multiple Emulsion (Expedited Award for Novel Research)

这项加速的探索性工程研究将评估利用一种新方法形成稳定的血红蛋白-油-水-盐溶液(Hb/o/w)多重乳剂用作人工血液的可行性。随着这一方法的发展，浓缩的血红蛋白溶液将在合适的无毒油相中乳化，然后将一次乳状液分散到含有适当无毒表面活性剂的外水相中形成多重乳状液。控制微滴的大小、包裹微滴的油界面膜的稳定性和厚度以及一次乳滴的大小，以实现氧气的快速传质。界面层(即一种液膜)的稳定性将通过测量界面流变性来评估。

This expedited exploratory engineering research study will evaluate the feasibility of utilizing a novel method to form stable hemoglobin-in-oil-aqueous-saline-solution (Hb/o/w) multiple emulsions for use as an artiffical blood. With the development of this methodolgy, a concentrated hemoglobin solution will be emulsified in a suitable nontoxic oil phase, followed by dispersion of the primary emulsion into an outer aqueous phase, containing suitable nontoxic surfactant to form the multiple emulsion. The size of the microdroplets, the stability and thickness of the oil interfacial membrane enveloping the microdroplets, and the size of the primary emulsion droplets will be controlled to achieve rapid mass transfer of oxygen. The stability of the interfacial layer (i.e., a type of liquid membrane) will be assessed by measuring interfacial rheological properties.