Response of Anchorage-Dependent Animal Cells to Fluid Shear Stress

最近，人们越来越感兴趣， 用于生产医学用的贴壁依赖性动物细胞 重要的生物化学品以及这些细胞的可能用途 人造器官。 这些细胞与体外 环境知之甚少。 本研究的目的是 检查锚定依赖性动物细胞对流体剪切力的反应 压力 平行板流动通道将被构建和测试 以将细胞暴露于良好限定的层流场。 细胞 从表面脱离以及细胞内的非致死性变化 行为和代谢（如渗透性增加， 形态学变化）和蛋白质分泌将作为 所施加的剪切应力的大小和持续时间的函数。 表面对细胞脱落和细胞内其他变化的影响 行为将通过研究对剪切应力的反应来检查， 在各种天然和合成表面上生长的细胞。

Recently, there has been increased interest in growing anchorage-dependent animal cells for the production of medically important biochemicals as well as for the possible use of these cells in artificial organs. The interaction of these cells with the in-vitro environment is poorly understood. The objective of this study is to examine the response of anchorage-dependent animal cells to fluid shear stresses. A parallel plate flow channel will be constructed and tested to expose the cells to a well-defined laminar flow field. Cell detachment from the surface as well as nonlethal changes in cell behavior and metabolism (such as increased permeability and morphological changes) and protein secretion will be examined as a function of the magnitude and duration of the applied shear stress. The effect of the surface on cell detachment and other changes in cell behavior will be examined by studying the response to shear stress of cells grown on a variety of natural and synthetic surfaces.