Sequestered Proton Domains in Chloroplast Bioenergetics

叶绿体能量学中分离的质子结构域：在蔗糖或氯化钾中重悬叶绿体会影响它们对单次翻转闪光激发和ATP形成的后续反应。这一突破性的观察结果将用于确定叶绿体中光合电子传递产生的质子梯度如何以及何时用于合成ATP以及影响这些反应的因素。自从大约15年前Peter Mitchell博士提出的质子梯度驱动ATP合成的化学渗透理论被普遍接受以来，一直存在争议，有人声称在体内条件下，局部质子域参与转导，有人声称只有质子在体相之间移动参与转导体内和体外。本研究的结果有可能解决争议，并确定每种机制的适用条件。所有生物使用的大部分生物能都是基于这个反应。因此，它成为我们生物能量知识的基石。

Sequestered Proton Domains in Chloroplast Energetics: Resuspending chloroplasts in sucrose or potassium chloride affects their subsequent response to single turnover flash excitation and to ATP formation. This breakthrough observation will be used to determine how and when proton gradients formed as a result of photosynthetic electron transport in chloropasts are used to synthesize ATP and the factors effecting these reactions. Since the chemosmotic theory of ATP synthesis driven by a proton gradient originally postulated by Dr. Peter Mitchell was generally accepted about 15 years ago there has been a controversy between those who claim that under in vivo conditions localized proton domains are involved in the transduction and those who claim that only protons moving between bulk phases are involved both in vivo and in vitro. The results of this research have the potential of settling the the controversy and defining the conditions under which each mechanism holds. Most of the biological energy used by all organisms is based on this reaction. It, therefore, becomes a keytstone of our bioenergetic knowledge.