Flow Cytometric Study of Ciliate Physiology

PI建议使用流式细胞仪收集以下信息： 纤毛虫的摄食、生长和繁殖模式 原生动物在不同的环境。 由此产生的信息 最终可用于废水处理设施， 并作为其他类似微生物的模型系统 在不同的生态系统中。 原生动物是单细胞的真核微生物， 消化细菌 流式细胞术不仅可以提供大量的 规模的统计信息，而且信息的 单个细胞的内部结构。 的具体目标 该项目包括： (1)生理指标的发展：生理指标的发展或测量 恒定环境中单个细胞的状态 (2)针对不断变化的环境扩展上述（1） (3)悬浮细胞饲养模型的建立 不同的生理状态， 适用于数量密度为 以及粒子群的组成 以不同的食物为食。 (4)开发一个数学模型， 细胞联合分布预测 纤毛虫种群的特性以及这些特性 分布变化响应于 环境条件 这两个PI都受到同行的尊重， 非常适合这项工作。

The PIs propose to use a flow cytometer to gather information on the feeding, growth, and reproduction patterns of ciliated protozoa in varying environments. The information thus generated could have ultimate use in wastewater treatment facilities as well as serve as a model system for other similar microorganisms in various ecological systems. Protozoa are unicellular, eucaryotic microorganisms that ingest and digest bacteria. Flow cytometry can not only provide large scale statistical information but also information on the internal structure of single cells. The specific objectives of the project include: (1) Development of an index or measure of the physiological state of a single cell in a constant environment (2) Extension of (1) above for changing environments (3) Development of a model of suspension feeding by cells of different physiological states that will be applicable to situations where the number density as well as the composition of the population of particles fed upon vary. (4) Development of a mathematical model that will allow the prediction of the joint distributions of cellular properties in a ciliate population and how these distributions change in response to changes in environmental conditions. Both PIs are well respected by their peers and the facilities are ideal for this work.