PostDoctoral Research Fellowships in Plant Biology

提出的研究使用可用的ligle特异性突变体来询问极化细胞分裂是如何调节的。该研究的目的是验证细胞骨架在指导细胞分裂的遗传信息传递中起结构作用的假设。该假设将被测试，表征正常和液泡特异性突变体的细胞骨架变化，使用完整和切片叶组织的免疫细胞化学。叶片原基的显微操作将测试叶舌的测定时间，抑制剂实验将测试特定细胞骨架成分的作用。DNA合成的时间，由放射自显影术确定，将把细胞骨架的变化置于细胞周期的背景下。由于舌瓣分裂是精确和可预测的，与突变植物的比较研究将提供一种分离分裂过程中特定控制点的方法。这些结果将阐明细胞骨架在分裂过程中参与建立细胞极性的过程。为了完全了解在发育中的有机体中导致有序程序的机制事件，有必要使用遗传学、细胞生物学、生物化学等技术。然而，单个实验室往往无法获得多种专业知识，因此没有使用综合方法。这个项目将遗传学和细胞生物学结合起来，提出一个重要的发育问题。如果通过这项研究，细胞骨架被认为是细胞中遗传信息的管道，那么其影响将在生物学的各个方面感受到，而不仅仅是植物生物学领域。

The proposed study uses the available ligule-specific mutants to ask how polarized cell divisions are regulated. The goal of the study is to test the hypothesis that the cytoskeleton plays a structural role in the transmission of the genetic information that directs cell division. The hypothesis will be tested characterizing the cytoskeletal changes of normal and ligule-specific mutants using immunocytochemistry of intact and sectioned leaf tissue. Micromanipulation of leaf primordia will test for the time of determination of the ligule, and inhibitor experiments will test for the role of specific cytoskeletal components. The time of DNA synthesis, as determined by autoradiography, will place the cytoskeletal changes within the context of the cell cycle. Since ligule division is precise and predictable, a comparative study with mutant plants will provide a means to isolate specific control points during division. These results will clarify the process by which the cytoskeleton participates in the establishment of cell polarity during division. In order to completely understand the mechanistic events which result in an ordered program in the developing organism, it is necessary to use the techniques of genetics, cell biology, biochemistry, etc. Too often, however, the several binds of expertise are not available to a single laboratory and the combined approach is not used. This project brings together genetics and cell biology to ask a significant developmental question. If the cytoskeleton is implicated as a conduit of genetic information in the cell by this study, the impact will be felt in all aspects of biology, not just the area of plant biology.