Enzymology of DNA Repair, SFC Award in Pakistani Currency

描述：本项目是为了研究硫代分枝杆菌中烷基化DNA暴露于烷基化剂后细胞修复的分子机制。这些实验将涉及酶的纯化和表征，这些酶似乎是某些烷基化试剂所特有的。为了研究修复蛋白和DNA碱基加合物之间的物理化学相互作用，将对它们的结构化学进行研究。这些实验将由巴基斯坦拉赫尔高级分子生物学中心(CAMB)的A.Athar博士进行。约翰斯·霍普金斯大学劳伦斯·格罗斯曼博士的实验室将协助巴基斯坦合作者进行那些在巴基斯坦无法完成的实验。范围：合作对两个机构都是有益的，并有望产生信息，从而更好地了解遗传物质烷基化损伤的细胞修复的分子机制。这两个实验室过去的工作已经产生了几个联合出版物。

Description: This project is for research on the molecular mechanisms of cellular repair of alkylated DNA in M. Iuteus after its exposure to alkylating agents. The experiments will involve purification and characterization of enzymes which are seemingly specific to certain alkylating agents. Their structural chemistry will be studied in order to investigate the physiochemical interaction between repair proteins and DNA base adducts. These experiments will be carried out by Dr. A. Athar at the Center for Advanced Molecular Biology (CAMB) in Lahere, Pakistan. The laboratory of Dr. Lawrence Grossman at Johns Hopkins University will assist Pakistani collaborators in conducting those experiments that could not be done in Pakistan. Scope: The collaboration is beneficial to both institutions and is expected to yield information leading to better understanding of molecular mechanisms of cellular repair of alkylation damage to genetic material. The past work by the two laboratories has resulted in several joint publications.