Neutron Scattering Analysis of Biological Structures: An External Users Program

中子散射的使用特别适合于蛋白质-核酸复合体和膜的分析，因为氢和氢之间的中子散射有较大的差异。这种差异使人们很容易区分出氚标记的蛋白质和未标记的蛋白质、脂类和核酸。在蛋白质结晶学中，中子以其错综复杂的氢键揭示了氢和/或氢原子的存在。氢-氚交换研究提供了有关蛋白质结构的动态性质的信息，并提供了特定电荷转移机制的详细描述。布鲁克海文中子散射设施为美国各地的生物学家提供了三个带有2D探测器的自动数据采集系统，用于蛋白质结晶学、膜衍射和小角散射。该设施还为设施的有效运行和维护提供了技术和科学人员，以及为不熟悉中子散射技术的用户提供“生物中子”专业知识。

The use of neutron scattering is particularly suited for the analysis of protein-nucleic acid complexes and membranes because of the relatively large difference in neutron scattering between hydrogen and deuterium. This difference makes it easy to distinguish deuterium labeled proteins from unlabeled proteins, lipids and nucleic acids. In protein crystallography, neutrons reveal the presence of hydrogen and/or deuterium atoms with their intricate hydrogen bonding. Hydrogen-deuterium exchange studies provide information on dynamic properties of protein structures and provide detailed description of specific charge transfer mechanisms. The Brookhaven Neutron Scattering Facilities provides three automatic data acquisition systems with 2D detectors for protein crystallography, membrane diffraction and small angle scattering for the use of biologists through the U.S. The facility also provides technical and scientific staff for efficient running and maintenance of the facilities, as well as provision of "biological neutron" expertise for users unfamiliar with neutron scattering techniques.