Production of Polyclonal and Monoclonal Antibodies for Detection of Sulfonamides

自1950年以来，畜禽饲料中抗生素和磺胺类药物的掺入量一直在稳步上升。在动物饲料中添加亚治疗性水平的抗菌剂正引起越来越多的关注，因为它可能对人类健康造成危害。目前磺胺类药物的分析方法费时费力，需要大量的样品制备。这项研究的目标是：(1)生产可作为分析试剂的单抗，以高特异性和敏感度检测样品中的磺胺类药物，只需很少或不需要清理；(2)成功构建稳定的细胞株，分泌所需的单抗。这种检测方法的成功开发将极大地促进肉类和家禽中磺胺类残留的检测，从而有助于确保美国人民的食品安全。

The incorporation of antibiotics and sulfonamides in livestock and poultry feeds has risen steadily since 1950. Concern over the addition of subtherapeutic levels of antimicrobials to animal feed is growing because of its potential as a hazard to human health. Current methods of analysis of sulfonamides are labor-intensive, time-consuming and require extensive sample preparation. The goals of this research are: (1) production of monoclonal antibodies which can serve as analytical reagents to detect, with high specificity and sensitivity, sulfonamides in samples requiring little or no cleanup, and (2) successful construction of stabilized cell lines which secrete the desired monoclonal antibodies. Successful development of such assays will greatly facilitate the detection of sulfonamide residues in in meat and poultry and thus help to assure the safety of food products for the American people.