Structure Function Relationships on Cytochrome c

该项目属于生命过程化学计划（COLP），由化学和生物物理联合支持。开展蛋白质结构-功能关系的基础研究。从酵母中提取的异细胞色素c用于研究氨基酸替换和缺失如何影响关键参数，如电子转移速率、结合强度、对细胞色素c蛋白伴侣的特异性和蛋白质稳定性。详细的细胞内测量将与体外工作进行比较，使用乳酸培养基中的生长作为相对比活性的测量。每项研究都产生了特定的突变。随着实验工作的开展，各种各样的理论方法正在被开发出来，以帮助设计和解释实验。该项目是先前在COLP倡议下支持的项目的延续。

This project is in the Chemistry of Life Processes Initiative (COLP) and supported jointly by Chemistry and Biophysics. Fundamental studies of protein structure-function relationships will be carried out. Iso-cytochrome c from yeast is used to study how amino acid replacements and deletions affect key parameters such as electron transfer rates, binding strength, specificity to protein partners of cytochrome c, and protein stability. Detailed intracellelar measurements will be made for comparison with the in vitro work, using growth in lactate medium as a measure of relative specific activity. Specific mutations have been generated for each of the studies. Along with the experimental work, a variety of theoretical approaches are being developed to aid in the design and interpretation of experiments. This project is a continuation of that previously supported under the COLP Initiative.