Lipase and Lipid Bodies in Seeds

这个项目的重点是三酰甘油在种子中的初始水解成脂肪酸。脂肪酶催化整个糖异生的第一步，因此可能是速率控制的，有证据表明它的作用受到内部和外部控制。被半单位膜包围的三酰甘油。在本项目中，将探索玉米脂肪酶与脂体之间的特异性结合。它们的识别信号将通过不同的生化方法和通过氨基酸序列分析蛋白质结构来研究。为了补充上述研究，还将进行蛋白质的定点突变，以精确定位结合位点。关于玉米的研究结果的一般模式将扩展到棉花脂肪酶。种子萌发和萌发后生长代表了生命周期的活跃和动态时期。该系统为新陈代谢和发育的研究提供了理想的机会。种子萌发中新陈代谢最突出的方面是动员食物储备以支持胚胎的生长。在种子的储存组织中，有三种主要的食物储备，即脂类、蛋白质和淀粉。大多数种子都含有相当多的脂类，谷类中例外。即使在谷物中，一些(种子重量的2%-5%)储存脂肪也存在于谷物的不同部分，这取决于物种和品种。脂肪在种子中是很好的食物储存，因为在单位体积的基础上，它们所拥有的能量大约是淀粉或蛋白质的两倍。储脂被动员起来，为胚轴的生长提供碳骨架和能量。该项目支持研究脂肪在种子中的利用。*/

This project focuses on the initial hydrolysis of triacylglycerols to fatty acids in seeds. Lipase catalyzes the first step in the overall gluconeogenesis and thus may be the rate-controlling, and there is evidence that its action is subjected to internal and external controls. The triacylglycerols surrounded by a half-unit membrane. In this project, the specific binding between the maize lipase and lipid bodies will be probed. Their recognition signals will be studied by different biochemical methods and by analyses of the protein structures via their amino acid sequences. Site-directed mutagenesis of the proteins will also be performed to complement the above studies in pinpointing the binding sites. The general pattern of the findings on maize will be extended to cotton lipases.%%% Seed germination and post germinative growth represent an active and dynamic period of the life cycle. The system offers an ideal opportunity for the study of metabolism and development. The most prominent aspect of metabolism in seed germination is the mobilization of food reserves to support the growth of the embryo. In the storage tissues of seeds, three major food reserves are present; namely, lipids, protein, and starch. Most seeds contain a sizable amount of lipids, with notable exception in cereal grains. Even in cereals, some (2-5% of seed weight) storage lipid is present in various parts of the grains, depending on the species and variety. Lipids are good food storage in seeds since on a per volume basis they possess about twice as much energy as starch or proteins. The storage lipid is mobilized to provide carbon skeleton and energy for growing embryonic axis. This project supports studies on how lipids are utilized in seeds.***//