Molecular Genetics of the PAS Region

胚胎决定的建立和维持 基因表达的差异模式对于 正常的动物发育。 在果蝇胚胎中 至少部分地，确定是在所述控制器的控制下。 双胸和触角足复合体的选择基因。 我 一直在研究一个基因区域， PAS-R，它包含许多相关基因。 适当的 这些基因的功能对正常发育至关重要 这似乎是适当监管 选择基因 该提案由分子和 PAS-R的发育遗传分析。 我建议 使用经典遗传学、体外诱变和生殖系 转化以严格鉴定单个蛋白质， 具有功能基因的RNA。 我将对cDNA克隆进行测序 从这些基因中提取氨基酸， 它们编码的蛋白质序列。 这也许能告诉我们 了解它们的功能，这是有价值的信息 用于制造蛋白质的抗体探针。 我建议 使用发育和分子方法来识别 其调节需要PAS-R基因存在的基因 产品. 将开发实验系统， 使我们能够研究这一规定，并确定 PAS-R蛋白的功能结构域与靶点识别 序列的 基因表达的编排导致了 成年人的发展在很大程度上仍不清楚。 深入了解基因的功能，如在 PAS-R区域被认为是调节 已知对发育至关重要的基因 从这项研究中。

The establishment and maintenance of embryonic determination and differential patterns of gene expression is essential for normal animal development. In Drosophila embryonic determination is, at least in part, under the control of the selector genes of the bithorax and Antennapedia complexes. I have been studying a genetic region, referred to as the PAS-R, that contains a number of related genes. The proper function of these genes is essential for normal development and it appears to be required for the proper regulation of selector genes. This proposal consists of a molecular and developmental genetic analysis of the PAS-R. I propose to use classical genetics, in vitro mutagenesis, and germ line transformation to rigorously identify individual proteins and RNAs with functional genes. I will sequence cDNA clones derived from these genes to determine the amino acid sequences of the proteins they encode. This may tell us something about their function, and is valuable information for making antibody probes for the proteins. I propose to use both developmental and molecular approaches to identify genes whose regulation requires the presence of PAS-R gene products. Experimental systems will be developed that will enable us to study this regulation, and to identify functional domains of PAS-R proteins and target recognition sequences. The orchestration of gene expression which results in the development of an adult is still largely not understood. Insight into the function of genes such as those found in the PAS-R region which are thought to regulate the expression of genes known to be developmentally essential should result from this study.