Analysis of DNA Sequence Recognition by Enzymes

总的目标是了解的结构基础， DNA结合蛋白的序列识别。 这些研究 第二类限制修改系统 特别是在Pvu II系统上， 之前从普通变形杆菌中克隆出来的 四种蛋白质， 识别包括Pvu II的相同DNA序列 蛋白质将被表征和比较。 该比较 将是结构性和功能性的，旨在 表明功能相关的蛋白质 识别一个共同的碱基序列显示结构同源性。 蛋白质对特定DNA序列的识别 对许多细胞过程至关重要， 了解细胞生理学将是可能的， 理解这种认知的基础。 这些过程 包括通过RNA聚合酶的启动子选择， 阻遏物，激活物，增强子调节，遗传 重组和转座以及靶向DNA修复。 博士 布卢门塔尔是训练有素的工作在这里描述。

The overall goal is to understand the structural basis of sequence recognition by DNA binding proteins. These studies have centered on the type II restriction-modification systems of bacteria, and in particular on the Pvu II system that was previously cloned from Proteus vulgaris. Four proteins that recognize the same DNA sequence which includes the Pvu II proteins will be characterized and compared. This comparison will be both structural and functional, and is intended to indicate the extent to which functionally-related proteins recognizing a common base sequences show structural homologies. The recognition of specific DNA sequences by proteins is so crucial to so many cellular processes that no mature understanding of cell physiology will be possible without understanding the basis of this recognition. These processes include promoter selection by RNA polymerases, the action of repressors, activators, enhancer-modulation, genetic recombination and transposition, and targeted DNA repair. Dr. Blumenthal is well trained for the work described here.