Molecular Mapping of the Chlamydomonas Genome

单细胞绿色衣藻莱茵衣藻具有 几个独特的特点，使其成为模型系统， 参与基因的分子遗传学研究的选择 真核生物鞭毛、叶绿体、细胞间相互作用， 一系列的生化途径。 我们将开发一种分子 衣原体基因组图谱，以补充广泛的 目前存在的基因图谱。 限制性片段长度 多态性，区分DNA从两个可 菌株将用于跟踪分子分离 遗传杂交中的标记。 分子标记将被绘制出来 关于19个基因组中每一个上的表型标记， 连锁群 连锁分子标记的集合将被排序 在每个链接组上。 将映射连接组的末端 使用端粒相关的分子标记。 精确 分子图谱和遗传图谱之间的相关性将 通过在一个区域进行“染色体漫步”来寻找 基因组 该项目将极大地促进分子和 衣原体核基因的遗传分析 在许多实验室中进行，包括我们自己的实验室。 单细胞的绿色披衣菌已经成为 研究许多问题的重要模式生物 基本的生物学兴趣。 这是一个建议， 绘制出详细而全面的DNA和基因图谱 这个有机体。 这样的地图将是一个宝贵的资源， 这将大大方便他们的工作。

The unicellular green alga Chlamydomonas reinhardtii has several unique characteristics that make it the model system of choice for molecular genetic studies of genes involved in eukaryotic flagella, chloroplasts, cell-cell interactions, and a number of biochemical pathways. We will develop a molecular map of the Chlamydomonas genome to complement the extensive genetic map that currently exists. Restriction fragment length polymorphism that distinguish the DNA from two interfertile strains will be used to follow the segregation of molecular markers in genetic crosses. Molecular markers will be mapped with respect to phenotypic markers on each of the nineteen linkage groups. Sets of linked molecular markers will be ordered on each linkage group. The ends of linkage groups will be mapped using molecular markers associated with telomere. Precise correlation between the molecular map and the genetic map will be sought by carrying out a "chromosome walk" in one region of the genome. This project will greatly facilitate molecular and genetic analysis of Chlamydomonas nuclear genes currently underway in many laboratories including our own. The single-celled green alga Chlamydomonas has become an important model organism for the study of a number of problems of fundamental biological interest. This is a proposal to develop a detailed and comprehensive map of the DNA and genes of this organism. Such a map will be a valuable resource to workers in the field, and will greatly facilitate their work.