Protein Sequence Instrumentation

本申请要求资金支持购买能够进行蛋白质和肽测序的仪器。这将成为多用户、多学科分子生物学设施的一部分。获得联邦拨款支持的8个核心用户将从中受益。基础生命科学学院不同学术部门的研究项目将使用该仪器进行研究：1。蛋白质在溶液和膜中的分子结构和功能2. 骨形成细胞中蛋白多糖的转化；3. 细胞内蛋白质转运与蛋白质工程；4. 心脏乙酰胆碱酯酶的表达调控7. 膜磷脂酰化的机制研究8. 病毒中的蛋白质- dna相互作用。所有项目都依赖于该仪器的可用性，该仪器能够快速可靠地对少量肽进行测序。

This application requests funds to support the purchase of instrumentation capable of carrying out protein and peptide sequencing. This will be part of a multiuser, multidisciplinary molecular biology facility. Eight core users with federal grant support will benefit. Research projects housed in different academic divisions of the School of Basic Life Sciences with the availability of this instrumentation will study: 1. Molecular structure and function of proteins in solution and in membranes; 2. Proteoglycan turnover in bone forming cells; 3. Intracellular protein traffic and protein engineering; 4. Regulation of expression of heart acetylcholinesterase; 7. Mechanism of acylation in membrane phospholipids; 8. Protein-DNA interaction in viruses. All projects depend upon the availability of this instrumentation capable of rapidly and reliably sequencing small amount of peptides.