Protein Sequence Instrumentation

该申请要求资金支持购买 能够进行蛋白质和肽的仪器 测序 这将是多用户的一部分， 多学科分子生物学设施。 八个核心 获得联邦拨款支持的用户将受益。 研究 项目安置在学校的不同学术部门 基础生命科学的发展， 仪器将研究：1。分子结构和 蛋白质在溶液和膜中的功能; 2. 骨形成细胞中的蛋白聚糖周转; 3.细胞内 蛋白质运输和蛋白质工程; 4.调控 心脏乙酰胆碱酯酶的表达; 7.机制 膜磷脂酰化; 8.蛋白质-DNA 病毒的相互作用。 所有项目都依赖于 该仪器的可用性能够快速和 可靠地测序少量肽。

This application requests funds to support the purchase of instrumentation capable of carrying out protein and peptide sequencing. This will be part of a multiuser, multidisciplinary molecular biology facility. Eight core users with federal grant support will benefit. Research projects housed in different academic divisions of the School of Basic Life Sciences with the availability of this instrumentation will study: 1. Molecular structure and function of proteins in solution and in membranes; 2. Proteoglycan turnover in bone forming cells; 3. Intracellular protein traffic and protein engineering; 4. Regulation of expression of heart acetylcholinesterase; 7. Mechanism of acylation in membrane phospholipids; 8. Protein-DNA interaction in viruses. All projects depend upon the availability of this instrumentation capable of rapidly and reliably sequencing small amount of peptides.