US-Belgium Cooperative Research: Regulation of Phage Mu Transposition

该奖项将支持一组美国和比利时生物化学家之间的合作研究。美国调查人员是阿拉巴马大学伯明翰分校的n·p·希金斯博士和科罗拉多大学健康科学中心的马丁·帕托博士。比利时研究人员是布鲁塞尔自由大学的阿丽亚娜·杜桑博士。该项目的长期目标是了解细菌种群中基因转位的调控。转座酶和半必需基因在体内参与高效的复制-转座反应。在这个__ ____项目中，将进行实验来分析在转录和DNA合成阶段影响Mu生物学的转座酶和早期基因。协作工作的目标包括i)表达，ii)纯化和iii)鉴定由病毒编码的DNA结合蛋白。研究人员将共同致力于大肠杆菌和_ ____鼠伤寒沙门氏菌的染色体相关蛋白（HU和其他“组蛋白样”蛋白）的研究。这种方法将扩展组织功能性折叠类核的染色体蛋白的基本知识__________ ___________。这三位研究人员管理的实验室在所有待研究的领域都显示出互补的专业知识。在__ _____ Birmingham建立了用于分析Mu转录和体外复制的纯化蛋白系统。在丹佛建立了分析Mu转录和体内复制的方法。布鲁塞尔的研究小组有选择突变体的遗传策略，并对与Mu生物学相关的蛋白质进行遗传解剖。通过在三个地点协调实验，研究人员可以在相对较短的_____ _______时间内从遗传学上测试一个假设，并在体外和体内测量预测的行为。

This award will support collaborative research between a group of U.S. and Belgian biochemists. The U.S. investigators are Dr. N. P. Higgins, University of Alabama at Birmingham and Dr. Martin Pato, University of Colorado Health Science Center. The Belgian researcher is Dr. Ariane Toussaint, Free University of Brussels. The long range goal of this project is to understand regulation of genetic transposition in bacterial populations. The Mu transposase and semi-essential genes participate in a highly efficient replication-transposition reaction in vivo. In this __ ____ project, experiments will be performed to analyze the transposase and early genes that influence Mu biology at the stages of the transcription and DNA synthesis. Objectives of the collaborative work include i) expression, ii) purification and iii) characterization of DNA binding proteins encoded by the virus. The investigators will extend joint efforts to chromosome-associated proteins (HU and other "histone-like" proteins) of E. coli and _ ____ Salmonella typhimurium. This approach will expand basic knowledge __________ ___________ of the chromosomal proteins that organize a functional folded nucleoid. The three investigators run laboratories that have demonstrated complementary expertise in all of the areas to be studied. Systems involving purified proteins for analyzing Mu transcription and replication in vitro are established in __ _____ Birmingham. Methods for analyzing Mu transcription and replication in vivo are established in Denver. The group in Brussels has genetic strategies for selecting mutants and carrying out the genetic dissection of proteins related to Mu biology. By coordinating experiments at the three sites, the investigators can test a hypothesis genetically and measure predicted behavior in ___ vitro and in vivo in a relatively short period of _____ _______ time.