PRF/M: Molecular Phylogeny of Loculoascomycetous Fungi

30多年前，子囊菌亚纲（子囊菌纲，真正真菌中的子囊菌纲）根据子囊间质和双舌子囊两种生殖特征的形态被定义。利用子囊基质的多个性状和单个子囊类型的组合，推导出了9500多个物种的隐含系统发育。目前对类群（特别是高等分类群）分类的分歧表明对潜在的单系类群缺乏清晰的认识。需要一个强大的系统发育，从中一套嵌套的单系群可以作为分类的来源。洛杉矶县自然历史博物馆的唐·r·雷诺兹博士将用分子数据来解决这个问题。研究将集中在32种房孢菌上，并将需要对相对较大的、进化保守的、连续重复的核糖体DNA进行测序。这种分析有望为研究祖先序列分化提供有用的数据。神经pora属将用于外群分析。聚合酶链反应（Polymerase Chain Reaction）和快速DNA测序方法将用于分析线粒体小亚基rDNA和细胞核rDNA，包括18s rRNA、28s rRNA、5.8s rRNA和基因间隔区基因。这项工作将于1989年9月在加州大学伯克利分校约翰·w·泰勒博士的实验室进行，为期12个月。

More than 30 years ago, the subclass Loculoascomycetes (of the Class Ascomycetes, among the true fungi) was defined on the basis of the morphology of two reproductive characters: the ascostroma and bitunicate ascus. The implied phylogeny has been derived for over 9500 species using a combination of multiple traits from the ascostroma and a single ascus type. Current disagreement over classification of the group (especially higher taxa) demonstrates the lack of a clear understanding of underlying monophyletic groups. A robust phylogeny from which a nested set of monophyletic groups can serve as the source of classification is needed. Dr. Don R. Reynolds, of the Natural History Museum of Los Angeles County, will address this issue with molecular data. Research will focus on 32 Loculoascomycete species and will entail sequencing the relatively large, evolutionally conservative and tandem repeated nuclear ribosomal DNA. Such analyses promise to yield useful data for study of ancestral sequence divergence. The genus Neuropora will be used in outgroup analysis. The Polymerase Chain Reaction coupled with rapid DNA sequencing methods will be used to analyze the small mitochnodrial subunit rDNA and the nuclear rDNA, including the genes for 18s rRNA, 28s rRNA, 5.8s rRNA, and the intergenic spacer region. The work will be carried out over a 12-month period beginning in September 1989 at the University of California, Berkeley, in the laboratory of Dr. John W. Taylor.