Metabolic Engineering of Aerobic Glucose Catabolism in Escherichia Coli

该项目旨在为大肠杆菌中心碳代谢的重大遗传重组提供重要框架，以增加有价值产品的合成。该项目还计划为研究细胞对环境或基因操纵的反应提供新的通用监测和分析方法。该项目的具体总体目标是通过对碳流进行基因改造来减少大肠杆菌有氧生长过程中醋酸盐的形成。这是通过识别代谢信号来完成的，这些信号是指导碳流向醋酸盐的候选信号。-在基因水平上识别与触发醋酸盐产生的环境反应相关的蛋白质。-评估总葡萄糖消耗，生长和醋酸酯形成动力学和化学计量，在导致醋酸酯生产的条件下。-详细描述具有或可能表现出醋酸酯生产代谢改变的几种现有遗传结构。-识别和绘制新的基因变化，以促进在产生醋酸盐的环境中生长。

This project intends to provide an important framework for major genetic restructuring of central carbon metabolism in E. coli for the purpose of increasing synthesis of valuable products. This project also plans to contribute to new monitoring and analysis methods of general utility in studying cell response to environmental or genetic manipulation. The specific overall objective of this project is to reduce acetate formation during aerobic growth of E. coli by genetic modification of carbon flow. This is to be done by: - Identifying metabolic signals which are candidates for directing carbon flow towards acetate. - Identifying proteins associated with responses at the genetic level to environments which trigger acetate production. - Evaluating overall glucose consumption, growth and acetate formation kinetics and stoichiometry under conditions which result in acetate production. - Characterizing in detail several existing genetic constructs which possess or which are likely to exhibit altered acetate production metabolism. - Identifying and mapping new genetic alterations which enhance growth in acetate-producing environments.