Identification of the Mouse Sperm Protein Responsible for Sperm-Egg Recognition

布莱尔博士的实验室的主要兴趣是在分子水平上分析哺乳动物受精，结合生物化学和分子遗传学的技术。这里提出的研究旨在确定两种已鉴定的小鼠精子蛋白中的哪一种具有ZP3识别蛋白的功能：即负责小鼠精子与透明带初级结合的精子蛋白。在初步研究中，根据亲和层析和交联性研究，已经鉴定出一种具有ZP3识别蛋白预期特性的小鼠精子“56K双重体”蛋白。这一蛋白质将进一步进行生化表征。也有人提出，与去唾液酸糖蛋白受体相关的精子半乳糖结合蛋白作为识别ZP3的小鼠精子蛋白发挥作用。这里描述的研究旨在确定这种大鼠蛋白是否与小鼠精子56kD蛋白有关，并独立地确定小鼠精子的去唾液酸糖蛋白受体相关蛋白是否具有ZP3识别蛋白预期的特性。拟议研究的长期目标是：1)鉴定和表征在透明带水平上调节精子和卵子之间相互作用的哺乳动物精子蛋白(受精蛋白“)；2)在分子水平上确定精子”受精蛋白“如何与透明带中的相应蛋白相互作用。这项工作建议识别精子上负责识别卵子表面受体的分子，这些受体使受精得以发生。%%%

The primary interest of Dr. Bleil's laboratory is analysis of mammalian fertilization at the molecular level, combining techniques of biochemistry and molecular genetics. Studies proposed here are designed to determine which of two identified mouse sperm proteins functions as the ZP3 recognition protein: i.e., the sperm protein responsible for primary binding of mouse sperm to the zone pellucida. In preliminary studies, a mouse sperm "56K doublet" protein having properties expected of the ZP3 recognition protein has been identified on the basis of affinity chromatography and crosslinking studies. This protein will be further characterized biochemically. It has been proposed by others that a sperm galactose-binding protein related to asialoglycoprotein receptor functions as the mouse sperm protein which recognizes ZP3. Studies described here are designed to determine whether this rat protein is related to the mouse sperm 56kD protein and, independently, whether an asialoglycoprotein receptor-related protein of mouse sperm has properties expected for the ZP3 recognition protein. The long term objectives of the proposed study are 1) to identify and characterize mammalian sperm proteins (fertilization proteins") which mediate interaction between sperm and egg at the level of the zona pellucida and 2) to determine, at the molecular level, how sperm "fertilization proteins" interact with their counterparts in the zone pellucida. %%% This work proposes to identify those molecules on sperm that are responsible for recognition of receptors on the egg surface that enable fertilization to take place. %%%