The Molecular Association of Complementary Adhesion Mole- cules Using Fluorescence Energy Transfer Spectroscopy

粘附淋巴细胞彼此或与其它细胞的粘附 是一个知之甚少的现象， 通过纯粹的定性和非物理方法。 解决 为此，主要研究者建议， 表征基础物理、化学和分子 在淋巴细胞转化过程中发生的相互作用 粘连 这一特性将通过以下方式实现： - 已知粘附的亲和常数的测量 分子。 - 变化率的量化 粘附过程中细胞间的接触面积 活动 荧光能量转移光谱技术， 视频显微镜和数字图像分析将被采用 分析结果将用于开发 淋巴细胞粘附的物理和数学模型 流程.

The adhesion of lymphocytes to one another or to other cells is a poorly understood phenomenon which is presently analyzed via purely qualitative and non-physical methods. To address this concern, the Principal Investigators propose to characterize fundamental physical, chemical, and molecular interactions that occur during the process of lyphocyte adhesion. This characterization will be achieved through: - Measurement of affinity constants of known adhesion molecules. - Quantification of the rate of change of the rate of change of the intercellular contact area during the adhesion event. Techniques in fluorescence energy transfer spectroscopy, video microscopy, and digital image analysis will be employed for this analysis and the results will be used to develop physical and mathematical models of lymphocyte adhesion processes.