US-Finland Cooperative Research on the Molecular Basis of Embryonic Induction

该奖项提供长期的部分支持 >（休假）研究访问教授斯科特F。吉尔伯特 斯沃斯莫尔学院到劳里·萨克森教授的实验室 赫尔辛基大学病理学系主任， 芬兰. 他们有兴趣了解早期的 形态发生的事件，并试图建立 哪些基因被诱导物激活了 他们的 一种方法是从未诱导的和 来自小鼠肾脏中新诱导的间充质组织。 通过 比较那些来自诱导和未诱导间充质的mRNA， 他们将能够确定哪些基因被激活， 诱导物，并建立一个时间和空间序列， 肾脏诱导 这正在成为一个强大的， 用于鉴定早期转录因子的超灵敏技术 事件 这次合作将萨克森博士的世界 胚胎肾诱导、分化 和形态发生与博士的经验和兴趣。 吉尔伯特在研究诱导的分子生物学方法 和组织能力。 发育生物学最大的挑战之一 关注核基因组如何指导形态发生 复杂的器官， 组织的排列。 这个问题的核心是细胞如何 响应来自相邻细胞的感应影响， 器官发生 哺乳动物肾脏的诱导 成为这种器官形成的模型， 两种组织之间的相互作用产生了一种 器官. 拟议的合作努力将有助于 我们对形态发生的分子基础的理解。

This award provides partial support for a long term >(sabbatical) research visit by Professor Scott F. Gilbert of Swarthmore College to the laboratory of Professor Lauri Saxen of the Department of Pathology of the University of Helsinki, Finland. They are interested in understanding the early events of morphogenesis, and are attempting to establish which genes are being activated by the inducer. Their approach is to construct cDNA libraries from uninduced and from newly induced mesenchymal tissue in mouse kidney. By comparing those mRNA's from induced and uninduced mesenchyme, they will be able to determine which genes are activated by the inducer and establish a temporal and spatial sequence of kidney induction. This is emerging as a powerful and ultasensitive techniques for identifying early transcriptive events. The collaboration brings together Dr. Saxen's world class expertise in embryonic kidney induction, differentiation and morphogenesis with the experience and interest of Dr. Gilbert in molecular biological methods for studying induction and tissue competence. One of the greatest challenges of developmental biology concerns how the nuclear genome instructs the morphogenesis of complex organs containing intricately coordinated arrangements of tissues. Central to this issue is how cells respond to inductive influences from neighboring cells during organogenesis. The induction of the mammalian kidney has become a model for such organogenesis where reciprocal interactions between two tissues create the rudiments of an organ. The proposed collaborative effort will contribute to our understanding of the molecular basis of morphogenesis.