Flow Cytometric Study of Ciliate Physiology

该计划旨在获得基本的工程信息 在摄食、生长和繁殖的动力学上， 梨形四膜虫 重点 观察单个细胞特性的分布， 开发模型来预测这种 分布变化响应于 环境条件 流式细胞术是 用于获得所需的主要实验工具 数据 这项技术可以快速准确地 确定单细胞的各种特性 共同分布在许多细胞中， 人口，它也可以用来提供 空间分布的定量信息 一些物质，比如DNA，在一个细胞中。

This program aims to obtain basic engineering information on the kinetics of feeding, growth, and reproduction of the organism Tetrahymena pyriformis. Emphasis is placed on observing distributions of single cell properties and developing models for prediction of how such distributions change in response to changes in environmental conditions. Flow cytometry is the principal experimental tool used to obtain the desired data. This technique allows rapid and accurate determination of how various properties of single cells are jointly distributed among the many cells in a population, and it can also be used to provide quantitative information on the spatial distribution of some materials, like DNA, in a single cell.