Genetic and Cell Biologic Studies of Mechanism of mRNA Exit from the Nucleus

本研究的目的是获得温度敏感的 酵母突变体，其中mRNA从细胞核的出口被阻断， 限制温度。 在选定的酵母诱变后 菌株、突变株将通过一系列筛选选出 旨在消除那些简单的程序， 对蛋白质合成或氨基酸摄取温度敏感， 并鉴定那些继续合成mRNA但 不允许它在不允许的情况下离开原子核 温度 虽然分子进出细胞核的运输 对细胞的生命至关重要， 这种运输是不被理解。 此外，尽管 信使RNA出口对所有真核生物的核心重要性 细胞，一种解决细胞这个基本问题的遗传学方法， 功能以前没有使用过。 成功获得 因此，温度敏感的信使RNA退出突变体将 提供重要的新工具， 这个过程的分子基础。

The goal of this research is to obtain temperature sensitive yeast mutants in which mRNA exit from the nucleus is blocked at the restrictive temperature. After mutagenesis of selected yeast strains, mutants will be selected through a series of screening procedures designed to eliminate those which are simply temperature sensitive for protein synthesis or amino acid uptake, and to identify those which continue to synthesize mRNA but do not allow it to exit the nucleus at the non-permissive temperature. Although transport of molecules into and out of the cell nucleus is critical for the life of a cell, the mechanisms regulating such transport are not understood. Furthermore, despite the central importance of messenger-RNA exit for all eukaryotic cells, a genetic approach to this fundamental problem of cell function has not previously been taken. Success in obtaining temperature sensitive messenger-RNA exit mutants would therefore provide important new tools that would permit analysis of the molecular basis of this process.