Mechanism of Metabolite Tranfer

在包括细胞中代谢序列的一系列反应中， 或者说是活的有机体，一个长期以来一直关注的核心问题是 生物化学家 是一种代谢产物，是一种酶的产物 反应和随后形成的酶促反应的底物 并在两种蛋白质的复合物中起作用， 在第一个系统中，然后游离到周围的环境中， 第二种媒介被捕获和利用？ 这个问题 将受到两种方式的攻击：（a）通过使用动态光 散射来研究组分的扩散性质 系统的单独和一起，并试图稳定 这两种蛋白质通过光化学反应 交联。 研究的两个系统将是甘油 磷酸脱氢酶和乳酸脱氢酶两种酶， 有一种常见的代谢物，烟腺嘌呤二核苷酸。

In the series of reactions comprising a metabolic sequence in cells or living organism, one central question has long concerned biochemists. Is a metabolite which is the product of one enzymic reaction and the substrate for a succeeding enzymic reaction formed and acted upon in a complex of the two proteins or is it produced in the first system and then dissociated into the surrounding medium to be captured and utilized by the second? This problem will be attacked in two ways: (a) by the use of dynamic light scattering to study the diffusional properties of the components of the system singly and together and by attempting to stabilize the presumptive complex of the two proteins through photochemical cross-linking. The two systems studied will be the glycerol phosphate dehydrogenase and lactate dehydrogenase two enzymes which have as a common metabolite, nicotinic-adenine-dinucleotide.