PRF/M: Ecological Genetics of Cuticle Permeability and Epi-cuticular Hydrocarbon Composition in Drosophila buzzatii and Thermobiology of Australian Cicadas (Homoptera:Cicadidae)

休假的主要目的是让 与斯图尔特·巴克博士合作的首席研究员 （澳大利亚阿米代尔的新英格兰大学） 角质层渗透性的生态遗传分析， 澳大利亚种群的表皮碳氢化合物组成 嗜仙人掌的果蝇，布氏果蝇。 内部和之间 这两个性状的群体遗传变异--以及它们的 塑性对环境温度和湿度变化的响应 制度-将使用半同胞实验量化 设计 这种分析将重复三次--在 每年夏季人口瓶颈，在恢复期间， 当人口规模达到最大时， G矩阵中可能的时间变化。 择偶实验 将评估表皮碳氢化合物在交配中的作用 系统D.布扎蒂。 辅助实验将量化 脱水对杜父鱼繁殖性能影响布扎蒂。 和巴克医生的休假将为我们提供 具有概念背景和遗传技术调查员 要求他进行自己的研究计划， 生理适应和驯化反应的演变 to environmental环境variation变化. 第二个项目--澳大利亚的实地和实验室研究 热生物学--将增强首席研究员的 与美国物种合作，并导致系统发育分析， 蝉的体温调节模式。

The primary purpose of the sabbatical is to allow the principal investigator to collaborate with Dr. Stuart Barker (University of New England, Armidale, Australia) on an ecological genetic analysis of cuticular permeability and epicuticular hydrocarbon composition in Australian populations of the cactiphilic fly, Drosophila buzzatii. Within and among population genetic variation for the two traits--and for their plastic response to variation in ambient temperature and humidity regimes--will be quantified using a half-sib experimental design. This analysis will repeated three times--during the annual summer population bottleneck, during recovery from the bottleneck, and when population sizes are maximal--to assess possible temporal changed in G-matrices. Mate choice experiments will assess the role of epicuticular hydrocarbons in the mating system of D. buzzatii. Ancillary experiments will quantify the impact of dehydration on reproductive performance of D. buzzatii. The sabbatical with Dr. Barker will provide the principal investigator with conceptual background and genetic techniques required to conduct his own program of research into the evolution of physiological adaptations and acclimation responses to environmental variation. A second project--field and laboratory studies o australian cicada thermobiology--will augment the principal investigator's work with U.S. species and lead to phylogenetic analysis of patterns of body temperature regulation in cicadas.